白色念珠菌与小鼠胃肠道黏膜的相互作用：辐射对体外黏附的影响
Interaction of Candida albicans with murine gastrointestinal mucosa: effect of irradiation on adherence in vitro.
作者信息
Sandovsky-Losica H, Segal E
机构信息
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
出版信息
J Med Vet Mycol. 1989;27(6):345-52.
The aim of this study was to investigate the adherence in vitro of Candida albicans to various parts of the gastrointestinal (GI) mucosa from irradiated and non-irradiated mice and to attempt to inhibit this adhesion with a chitin derivative. Adhesion was assayed using 3H-leucine labelled yeast, to which GI tissue-disks removed from irradiated (400R Cobalt) and non-irradiated animals were exposed at various time intervals post-irradiation. In non-irradiated mice differences in adherence of C. albicans to various parts of the GI tract were observed, the highest adherence being to duodenal tissues. In irradiated mice, an increase in adherence to all parts of the GI mucosa was observed. Based on findings from previous studies that a chitin derivative (CSE) inhibits adhesion of C. albicans to various tissues in vitro and in vivo, we tested the effect of CSE on the adhesion of C. albicans to GI tissues. The results show that CSE inhibited the adhesion of C. albicans to GI tissues from both irradiated and non-irradiated mice by 75-85%. The relevance of the findings to the pathogenesis of candidiasis is discussed.
本研究旨在调查白色念珠菌在体外对受辐照和未受辐照小鼠胃肠道（GI）黏膜各部位的黏附情况，并尝试用几丁质衍生物抑制这种黏附。使用³H-亮氨酸标记的酵母来测定黏附情况，将从受辐照（400R钴）和未受辐照动物身上取下的GI组织圆盘在辐照后的不同时间间隔暴露于该酵母。在未受辐照的小鼠中，观察到白色念珠菌对胃肠道各部位的黏附存在差异，对十二指肠组织的黏附最高。在受辐照的小鼠中，观察到对GI黏膜所有部位的黏附增加。基于先前研究发现几丁质衍生物（CSE）在体外和体内均能抑制白色念珠菌对各种组织的黏附，我们测试了CSE对白色念珠菌黏附GI组织的影响。结果表明，CSE可将白色念珠菌对受辐照和未受辐照小鼠GI组织的黏附抑制75 - 85%。本文讨论了这些发现与念珠菌病发病机制的相关性。
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