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法医生物学实验室实际操作中DNA转移情况的观察

Observations of DNA transfer within an operational Forensic Biology Laboratory.

作者信息

Taylor Duncan, Abarno Damien, Rowe Emily, Rask-Nielsen Lauren

机构信息

Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia; School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.

Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia; School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.

出版信息

Forensic Sci Int Genet. 2016 Jul;23:33-49. doi: 10.1016/j.fsigen.2016.02.011. Epub 2016 Feb 26.

DOI:10.1016/j.fsigen.2016.02.011
PMID:26977932
Abstract

Advances in technology to both generate and interpret DNA profiles has seen the expansion of the ability to provide opinions about results obtained from very low levels of starting biological material. The response in court has been to question the mode by which the DNA came to be on an item, rather than questioning its presence. This brings into play a number of real-world aspects such as transfer of biological material, persistence of biological material on items, shedding ability of individuals, just to name a few. There have been a number of studies that investigate different aspects relating the mode of DNA deposition and transfer, mostly under tightly controlled conditions. We add to this knowledge pool by investigating the extent to which individuals at Forensic Science SA (FSSA) deposit their DNA on objects throughout the floor of the building where DNA examinations take place. We find that the results obtained in our minimally controlled study allow us to comment on a number of published concepts.

摘要

在生成和解读DNA图谱的技术方面取得的进展,使得人们能够针对从极低起始生物样本量获得的结果提供意见的能力得到了扩展。法庭上的回应是质疑DNA出现在某一物品上的方式,而非质疑其存在与否。这就涉及到了一些现实世界的因素,比如生物样本的转移、生物样本在物品上的持久性、个体的脱落能力等等。已经有许多研究在严格控制的条件下,对与DNA沉积和转移方式相关的不同方面进行了调查。我们通过调查南澳大利亚法医科学研究所(FSSA)的人员在进行DNA检测的大楼整个楼层的物体上留下其DNA的程度,来丰富这一知识宝库。我们发现,在我们控制程度最低的研究中获得的结果,使我们能够对一些已发表的概念进行评论。

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