脊髓谷氨酸转运体参与电针耐受的形成。
Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance.
作者信息
Cui Luying, Ding Yi, Zeng Jie, Feng Yan, Li Meng, Ding Mingxing
机构信息
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
出版信息
Int J Mol Sci. 2016 Mar 10;17(3):357. doi: 10.3390/ijms17030357.
BACKGROUND
Electroacupuncture (EA) tolerance is a gradual decline in EA antinociception because of its repeated or prolonged use. This study aims to explore the role of spinal glutamate transporters (GTs) in EA tolerance (EAT).
METHODS
Rats were treated with EA once per day for eight consecutive days, and their L4-5 spinal cords were collected at days 0, 2, 4, 6 and 8. The levels of three spinal GTs and their mRNAs were detected with Western blot and pPCR, respectively. Then, riluzole, a positive GT regulator, was administered intrathecally in order to observe its effect on EA analgesia after repeated EA.
RESULTS
The expression levels of the spinal GTs increased at days 2 and 4, and gradually decreased as the times of EA increased. At day 8, no difference was observed in the spinal GTs between the sham treatment and the EA treatment. Intrathecal administration of riluzole dose-dependently attenuated the decreased EA analgesia.
CONCLUSION
These results indicated the participation of the spinal GTs in EAT.
背景
电针耐受是由于电针的反复或长时间使用导致其抗伤害感受作用逐渐下降。本研究旨在探讨脊髓谷氨酸转运体（GTs）在电针耐受（EAT）中的作用。
方法
大鼠连续8天每天接受一次电针治疗，分别在第0、2、4、6和8天采集其L4-5脊髓。分别采用蛋白质免疫印迹法和聚合酶链反应（pPCR）检测三种脊髓GTs及其mRNA的水平。然后，鞘内注射利鲁唑（一种GT阳性调节剂），以观察其对重复电针后电针镇痛效果的影响。
结果
脊髓GTs的表达水平在第2天和第4天升高，并随着电针次数的增加而逐渐降低。在第8天，假处理组和电针处理组之间的脊髓GTs没有差异。鞘内注射利鲁唑可剂量依赖性地减轻电针镇痛作用下降的程度。
结论
这些结果表明脊髓GTs参与了电针耐受。
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