Gupta Snehil, Ajith Kumar K G, Sharma Anil Kumar, Nagar Gaurav, Kumar Sachin, Saravanan B C, Ravikumar Gandham, Ghosh Srikant
Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India.
Exp Appl Acarol. 2016 Jun;69(2):239-48. doi: 10.1007/s10493-016-0032-7. Epub 2016 Mar 15.
Monitoring of acaricide resistance is considered as one of the important facets of integrated tick management. In an attempt of development of resistance monitoring indicators, in the present study two reference tick lines of Rhipicephalus (Boophilus) microplus maintained in the Entomology laboratory, Indian Veterinary Research Institute (IVRI), Izatnagar, India, were studied to determine the possible contributing factors involved in development of resistance to deltamethrin. Electrophoretic profiling of esterase enzymes detected high activities of EST-1 in reference resistant tick colony designated as IVRI-IV whereas it was not detectable in reference susceptible IVRI-I line of R. (B.) microplus. Esterases were further characterized as carboxylesterase or acetylcholinesterase based on inhibitor study using PMSF, eserine sulphate, malathion, TPP and copper sulphate. It was concluded that an acetylcholinesterase, EST-1, possibly plays an important role for development of deltamethrin resistance in IVRI-IV colony of R. (B.) microplus.
杀螨剂抗性监测被认为是蜱虫综合管理的重要方面之一。为了开发抗性监测指标，在本研究中，对印度伊扎特纳加尔印度兽医研究所（IVRI）昆虫学实验室保存的微小扇头蜱（Rhipicephalus (Boophilus) microplus）的两个参考蜱虫品系进行了研究，以确定与溴氰菊酯抗性发展相关的可能影响因素。酯酶的电泳分析检测到在指定为IVRI-IV的参考抗性蜱虫群体中EST-1活性较高，而在微小扇头蜱的参考敏感IVRI-I品系中未检测到。基于使用苯甲基磺酰氟（PMSF）、硫酸毒扁豆碱、马拉硫磷、三苯基膦（TPP）和硫酸铜的抑制剂研究，酯酶进一步被鉴定为羧酸酯酶或乙酰胆碱酯酶。得出的结论是，一种乙酰胆碱酯酶EST-1可能在微小扇头蜱IVRI-IV群体对溴氰菊酯抗性的发展中起重要作用。
