Wang Bao-Jian, Gu Xiao-Bin, Yang Guang-You, Wang Tao, Lai Wei-Min, Zhong Zhi-Jun, Liu Guo-Hua
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province 611133, PR China.
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province 611133, PR China.
Infect Genet Evol. 2016 Jun;40:228-235. doi: 10.1016/j.meegid.2016.03.012. Epub 2016 Mar 12.
Heterakis gallinae and Heterakis beramporia are the most prevalent nematode infecting native chicken breed, causing major economic losses. In the present study, the complete mitochondrial genomes (mt) of H. gallinae and H. beramporia were amplified by long-PCR and then sequenced. The complete mt genomes of H. gallinae and H. beramporia were 13,973bp and 14,012bp in size, respectively. Both mt genomes contain 12 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. All genes are transcribed in the same direction and the gene arrangement is identical to Ascaridia spp. Phylogenetic analysis based on the 12 protein-coding genes revealed that the family Heterakidae (represented by H. gallinae and H. beramporia) was more closely related to the infraorder Ascaridomorpha than it was to the infraorder Oxyuridomorpha. The present study determined the complete mt genome sequences for two Heterakis species, providing useful markers for studying the systematics, population genetics, and molecular epidemiology of these Heterakis parasites.
鸡异刺线虫和贝氏异刺线虫是感染本地鸡品种最为普遍的线虫，会造成重大经济损失。在本研究中，通过长链聚合酶链式反应扩增了鸡异刺线虫和贝氏异刺线虫的完整线粒体基因组（mt），随后进行测序。鸡异刺线虫和贝氏异刺线虫的完整线粒体基因组大小分别为13,973bp和14,012bp。两种线粒体基因组均包含12个蛋白质编码基因、22个转运RNA基因和2个核糖体RNA基因。所有基因均按相同方向转录，且基因排列与禽蛔线虫属相同。基于12个蛋白质编码基因的系统发育分析表明，异刺科（以鸡异刺线虫和贝氏异刺线虫为代表）与蛔形亚目相比，与尖尾亚目关系更为密切。本研究确定了两种异刺线虫物种的完整线粒体基因组序列，为研究这些异刺线虫寄生虫的系统学、群体遗传学和分子流行病学提供了有用的标记。
