Seppälä Miia, Halme Elina, Tiilikainen Lauri, Luukkainen Annika, Laranne Jussi, Rautiainen Markus, Huhtala Heini, Paavonen Timo, Toppila-Salmi Sanna
a Haartman Institute, University of Helsinki , Helsinki , Finland ;
b Department of Otorhinolaryngology , Tampere University Hospital, Tampere , Finland ;
Acta Otolaryngol. 2016 Jul;136(7):729-35. doi: 10.3109/00016489.2016.1152631. Epub 2016 Mar 16.
Conclusion IDO might be useful for predicting progression of primary tumor stage T2 and T3 in tongue squamous cell carcinoma (TSCC), but does not seem like a specific biomarker for diagnosing TSCC and predicting patient survival. Objectives Indoleamine 2,3-dioxygenase (IDO) is expressed in many cells and it catabolises the essential amino acid tryptophan to kynurenine. IDO acts as an immune modulator through suppression of T-cell immunity and other pathways. In cancer cells, IDO has been proposed to promote tumor progression by enabling malignant cells to escape from the immune system. The aim of this study was to evaluate the association and prognostic relevance of IDO expression in TSCC. Method One hundred and eight retrospective tongue and lymph node specimens were stained immunohistochemically with monoclonal antibody anti-indoleamine 2,3-dioxygenase. The relative abundance of IDO positive epithelial cells, IDO staining intensity, and inflammation were assessed semi-quantitatively with light microscopy. Results IDO was expressed stronger in tongue hyperplasia than in TSCC. However, IDO expression associated with poor survival in the sub-groups with primary tumor stage T2-T4 and in the sub-group with strong inflammation in tumors' invasive front.
结论 吲哚胺2,3-双加氧酶（IDO）可能有助于预测舌鳞状细胞癌（TSCC）中原发肿瘤T2和T3期的进展，但似乎不是诊断TSCC和预测患者生存的特异性生物标志物。目的 吲哚胺2,3-双加氧酶（IDO）在许多细胞中表达，它将必需氨基酸色氨酸分解代谢为犬尿氨酸。IDO通过抑制T细胞免疫和其他途径发挥免疫调节作用。在癌细胞中，有人提出IDO通过使恶性细胞逃避免疫系统来促进肿瘤进展。本研究的目的是评估IDO在TSCC中的表达与预后的相关性。方法 用抗吲哚胺2,3-双加氧酶单克隆抗体对108例回顾性舌和淋巴结标本进行免疫组织化学染色。用光学显微镜对IDO阳性上皮细胞的相对丰度、IDO染色强度和炎症进行半定量评估。结果 IDO在舌增生中的表达比在TSCC中更强。然而，在原发肿瘤T2 - T4期亚组以及肿瘤浸润前沿有强烈炎症的亚组中，IDO表达与较差的生存率相关。
