巴西东北部驴中抗新孢子虫属和肉孢子虫抗体的流行情况。

Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil.

作者信息

Gennari Solange Maria, Pena Hilda Fátima de Jesus, Lindsay David Scott, Lopes Marcos Gomes, Soares Herbert Sousa, Cabral Aline Diniz, Vitaliano Sérgio Netto, Amaku Marcos

机构信息

Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.

Center for Molecular Medicine and Infectious Disease, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA, USA.

出版信息

Rev Bras Parasitol Vet. 2016 Jan-Mar;25(1):109-11. doi: 10.1590/S1984-29612016003. Epub 2016 Mar 11.

DOI:10.1590/S1984-29612016003

PMID:26982557

Abstract

Sarcocystis neurona and Neospora hughesi are coccidian protozoa that can cause neurological illness in horses in America. In this study we report seroprevalence of Neospora spp. andS. neurona in sera of 333 donkeys from the northeastern region of Brazil. Antibodies to Neospora spp. were detected in 2% (7 donkeys) of 333 sera tested by the indirect fluorescent antibody test (IFAT) with a cut-off dilution of 1:40. Antibodies to S. neurona were found in 3% (10 donkeys) of the samples tested by IFAT (cut-off ≥50) and 21% (69 donkeys) by the direct agglutination test (SAT ≥50). The SAT and IFAT results for S. neurona showed a poor concordance (value of Kappa=0.051). This is the first report of Neospora spp. antibodies in Brazilian donkeys and the first detection of antibodies against S. neurona in this animal species.

摘要

肉孢子虫属神经元种和休氏新孢子虫是球虫原虫，可在美国导致马匹出现神经疾病。在本研究中，我们报告了巴西东北部地区333头驴血清中neospora属和肉孢子虫属神经元种的血清流行率。通过间接荧光抗体试验（IFAT），在333份检测血清中，有2%（7头驴）检测到针对neospora属的抗体，截断稀释度为1:40。通过IFAT（截断值≥50）检测的样本中，有3%（10头驴）检测到针对肉孢子虫属神经元种的抗体，通过直接凝集试验（SAT≥50）检测的样本中有21%（69头驴）检测到该抗体。肉孢子虫属神经元种的SAT和IFAT结果显示一致性较差（卡帕值=0.051）。这是巴西驴中首次报告neospora属抗体，也是首次在该动物物种中检测到针对肉孢子虫属神经元种的抗体。