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本文引用的文献

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Seroprevalence of Toxoplasma gondii infection in domestic horses in Durango State, Mexico.墨西哥杜兰戈州家养马中弓形虫感染的血清阳性率
J Parasitol. 2012 Oct;98(5):944-5. doi: 10.1645/GE-3174.1. Epub 2012 May 4.
2
Detection of Neospora sp. antibodies in cart horses from urban areas of Curitiba, Southern Brazil.巴西南部库里蒂巴市区役用马中新型隐孢子虫抗体的检测
Rev Bras Parasitol Vet. 2012 Jan-Mar;21(1):68-70. doi: 10.1590/s1984-29612012000100014.
3
Detection of antibodies against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii in horses from Costa Rica.哥斯达黎加马匹中抗新孢子虫、新孢子虫属和刚地弓形虫抗体的检测。
J Parasitol. 2011 Jun;97(3):522-4. doi: 10.1645/GE-2722.1. Epub 2011 Feb 10.
4
Improved detection of equine antibodies against Sarcocystis neurona using polyvalent ELISAs based on the parasite SnSAG surface antigens.利用基于寄生虫 SnSAG 表面抗原的多价 ELISA 提高对 Sarcocystis neurona 的马抗体的检测。
Vet Parasitol. 2011 Feb 28;176(1):16-22. doi: 10.1016/j.vetpar.2010.10.034. Epub 2010 Nov 13.
5
Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic.捷克共和国马匹中新型隐孢子虫和刚地弓形虫抗体。
Parasitol Res. 2010 Sep;107(4):783-5. doi: 10.1007/s00436-010-1929-4. Epub 2010 Jun 8.
6
Prevalence of Sarcocystis neurona and Neospora spp. infection in horses from Brazil based on presence of serum antibodies to parasite surface antigen.基于对寄生虫表面抗原的血清抗体存在情况,巴西马匹中肉孢子虫属神经元种和新孢子虫属感染的患病率
Vet Parasitol. 2006 Mar 15;136(2):155-9. doi: 10.1016/j.vetpar.2005.10.023. Epub 2005 Nov 28.
7
Recombinant NhSAG1 ELISA: a sensitive and specific assay for detecting antibodies against Neospora hughesi in equine serum.重组NhSAG1酶联免疫吸附测定:一种用于检测马血清中抗休氏新孢子虫抗体的灵敏且特异的检测方法。
J Parasitol. 2005 Apr;91(2):446-52. doi: 10.1645/GE-395R.
8
Risk of postnatal exposure to Sarcocystis neurona and Neospora hughesi in horses.马匹产后暴露于肉孢子虫属神经元种和休斯新孢子虫的风险。
Am J Vet Res. 2004 Aug;65(8):1047-52. doi: 10.2460/ajvr.2004.65.1047.
9
Seroprevalence of antibodies to Sarcocystis neurona in equids residing in Oklahoma.
J Vet Diagn Invest. 2003 Nov;15(6):597-600. doi: 10.1177/104063870301500617.
10
Prevalence of antibodies to Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii in wild horses from central Wyoming.怀俄明州中部野马中犬新孢子虫、神经肉孢子虫和刚地弓形虫抗体的流行情况。
J Parasitol. 2003 Aug;89(4):716-20. doi: 10.1645/GE-66R.

墨西哥马匹中抗马内氏肉孢子虫和休氏新孢子虫抗体的流行情况。

Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico.

作者信息

Yeargan Michelle R, Alvarado-Esquivel Cosme, Dubey Jitender P, Howe Daniel K

机构信息

Department of Veterinary Science, M.H. Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky 40546-0099, USA.

出版信息

Parasite. 2013;20:29. doi: 10.1051/parasite/2013029. Epub 2013 Sep 10.

DOI:10.1051/parasite/2013029
PMID:24016396
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3767921/
Abstract

Equine protozoal myeloencephalitis (EPM) is a debilitating disease of horses caused by Sarcocystis neurona and Neospora hughesi. Sera from 495 horses in Durango State, Mexico were tested for anti-protozoal antibodies using enzyme-linked immunosorbent assays (ELISAs) based on major surface antigens of these two parasites. Antibodies to S. neurona were detected in 240 (48.5%) of the 495 horse sera tested with the rSnSAG2/4/3 trivalent ELISA. Multivariate analysis showed that exposure to S. neurona was associated with age, feeding grains and crops, and small herd size. Antibodies to N. hughesi were found in 15 (3.0%) of the 495 horse sera tested with the rNhSAG1 ELISA and confirmed by Western blot of N. hughesi tachyzoite antigen. This is the first report of S. neurona and N. hughesi exposure in horses in Mexico, and it affirms that EPM should be in the differential diagnosis for horses exhibiting signs of neurologic disease in this country.

摘要

马属动物原虫性脑脊髓炎(EPM)是一种由神经肉孢子虫和休氏新孢子虫引起的马的衰弱性疾病。使用基于这两种寄生虫主要表面抗原的酶联免疫吸附测定(ELISA)对墨西哥杜兰戈州495匹马的血清进行抗寄生虫抗体检测。在用rSnSAG2/4/3三价ELISA检测的495份马血清中,有240份(48.5%)检测到了抗神经肉孢子虫抗体。多变量分析表明,接触神经肉孢子虫与年龄、喂食谷物和农作物以及小群体规模有关。在用rNhSAG1 ELISA检测的495份马血清中,有15份(3.0%)检测到了抗休氏新孢子虫抗体,并通过对休氏新孢子虫速殖子抗原的蛋白质印迹法得到证实。这是墨西哥马匹接触神经肉孢子虫和休氏新孢子虫的首次报告,并且证实了在该国出现神经疾病症状的马匹的鉴别诊断中应考虑EPM。