墨西哥马匹中抗马内氏肉孢子虫和休氏新孢子虫抗体的流行情况。
Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico.
作者信息
Yeargan Michelle R, Alvarado-Esquivel Cosme, Dubey Jitender P, Howe Daniel K
机构信息
Department of Veterinary Science, M.H. Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky 40546-0099, USA.
出版信息
Parasite. 2013;20:29. doi: 10.1051/parasite/2013029. Epub 2013 Sep 10.
Equine protozoal myeloencephalitis (EPM) is a debilitating disease of horses caused by Sarcocystis neurona and Neospora hughesi. Sera from 495 horses in Durango State, Mexico were tested for anti-protozoal antibodies using enzyme-linked immunosorbent assays (ELISAs) based on major surface antigens of these two parasites. Antibodies to S. neurona were detected in 240 (48.5%) of the 495 horse sera tested with the rSnSAG2/4/3 trivalent ELISA. Multivariate analysis showed that exposure to S. neurona was associated with age, feeding grains and crops, and small herd size. Antibodies to N. hughesi were found in 15 (3.0%) of the 495 horse sera tested with the rNhSAG1 ELISA and confirmed by Western blot of N. hughesi tachyzoite antigen. This is the first report of S. neurona and N. hughesi exposure in horses in Mexico, and it affirms that EPM should be in the differential diagnosis for horses exhibiting signs of neurologic disease in this country.
马属动物原虫性脑脊髓炎（EPM）是一种由神经肉孢子虫和休氏新孢子虫引起的马的衰弱性疾病。使用基于这两种寄生虫主要表面抗原的酶联免疫吸附测定（ELISA）对墨西哥杜兰戈州495匹马的血清进行抗寄生虫抗体检测。在用rSnSAG2/4/3三价ELISA检测的495份马血清中，有240份（48.5%）检测到了抗神经肉孢子虫抗体。多变量分析表明，接触神经肉孢子虫与年龄、喂食谷物和农作物以及小群体规模有关。在用rNhSAG1 ELISA检测的495份马血清中，有15份（3.0%）检测到了抗休氏新孢子虫抗体，并通过对休氏新孢子虫速殖子抗原的蛋白质印迹法得到证实。这是墨西哥马匹接触神经肉孢子虫和休氏新孢子虫的首次报告，并且证实了在该国出现神经疾病症状的马匹的鉴别诊断中应考虑EPM。
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