Franken Carmen, Remy Sylvie, Lambrechts Nathalie, Hollanders Karen, Den Hond Elly, Schoeters Greet
a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;
b Department of Biomedical Sciences ;
Biomarkers. 2016 Jul;21(5):458-65. doi: 10.3109/1354750X.2016.1153721. Epub 2016 Mar 17.
A crucial challenge for gene expression analysis in human biomonitoring studies on whole blood samples is rapid sample handling and mRNA stabilization. This study was designed to evaluate the impact of short bench times (less than 30 min) on yield, quality and gene expression of mRNA in the presence of different stabilization buffers (Tempus(TM) Blood RNA tube and RNAlater(®) Stabilization Reagent). Microarray analyzes showed significant changes over short periods of time in expression of a considerate part of the transcriptome (2356 genes) with a prominent role for NFкB-, cancer- and glucocorticoid-mediated networks, and specifically interleukin-8 (IL-8). These findings suggest that even short bench times affect gene expression, requiring to carry out blood collection in a strictly standardized way.
在全血样本的人体生物监测研究中，基因表达分析面临的一个关键挑战是快速的样本处理和mRNA稳定化。本研究旨在评估在不同稳定缓冲液（Tempus™血液RNA管和RNAlater®稳定试剂）存在的情况下，短时间放置（少于30分钟）对mRNA产量、质量和基因表达的影响。微阵列分析显示，在短时间内，转录组中相当一部分（2356个基因）的表达发生了显著变化，其中NFкB、癌症和糖皮质激素介导的网络，特别是白细胞介素-8（IL-8）发挥了重要作用。这些发现表明，即使是短时间放置也会影响基因表达，这就要求以严格标准化的方式进行血液采集。
