Wang Beilei, Liu Zhenzhen, Ma Zhao, Li Minyong, Du Lupei
Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
ACS Med Chem Lett. 2016 Jan 20;7(3):245-9. doi: 10.1021/acsmedchemlett.5b00360. eCollection 2016 Mar 10.
The detection and imaging of hERG potassium channels in living cells can provide useful information for hERG-correlation studies. Herein, three small-molecule fluorescent probes, based on the potent hERG channel inhibitor astemizole, for the imaging of hERG channels in hERG-transfected HEK293 cells (hERG-HEK293) and human colorectal cancer cells (HT-29), are described. These probes are expected to be applied in the physiological and pathological studies of hERG channels.
活细胞中hERG钾通道的检测与成像可为hERG相关性研究提供有用信息。本文描述了三种基于强效hERG通道抑制剂阿司咪唑的小分子荧光探针，用于在转染hERG的HEK293细胞（hERG-HEK293）和人结肠癌细胞（HT-29）中对hERG通道进行成像。这些探针有望应用于hERG通道的生理和病理研究。
