Strauss J, Mrázová M, Svandová E, Kokesová O
Cesk Epidemiol Mikrobiol Imunol. 1989 Dec;38(6):348-54.
The authors present the results of examination of 121 probands by the rubella test of radial haemolysis (RH), using capillary blood (direct test), as compared with the use of serum from venous blood in correlation with the rubella haemagglutination inhibition test. The diameter of the haemolytic zone, 7 mm, was as compared with the haemagglutination inhibition test in both modifications in 95% and the diameter 8 mm was seropositive in 100%. Haemolytic zones up to 6 mm must be considered seronegative even when some of the bloods or sera have bonder line haemagglutination inhibition titres. Seronegativity was expressed in the direct test of radial haemolysis by larger haemolytic zones. The specificity of the direct test of radial haemolysis, even when serum was used, was 87.5%, the sensitivity 88.2% and 88.4% resp. The reliability of positive diameters of haemolytic zones (7 mm+) is 99%, while that of negative ones (2-6 mm) only 35%. The less laborious direct test of RH proved equivalent to the radial haemolysis test using serum, as far as screening with the purpose of aimed immunization against rubella is concerned.
作者介绍了采用毛细血管血进行放射状溶血风疹试验(RH)对121名先证者进行检测的结果(直接试验),并将其与使用静脉血血清并结合风疹血凝抑制试验的结果进行了比较。溶血区直径为7mm时,在两种方法中与血凝抑制试验相比,95%的结果相符;直径为8mm时,100%的结果为血清学阳性。即使某些血液或血清的血凝抑制滴度处于临界值,溶血区直径达6mm及以下也应视为血清学阴性。在放射状溶血直接试验中,溶血区较大表示血清学阴性。放射状溶血直接试验,即使使用血清,特异性为87.5%,敏感性分别为88.2%和88.4%。溶血区阳性直径(7mm及以上)的可靠性为99%,而阴性直径(2 - 6mm)的可靠性仅为35%。就风疹目标免疫筛查而言,操作相对简便的RH直接试验被证明与使用血清的放射状溶血试验等效。