Rosenberg S A, Schwarz S, Anding H, Hyatt C, Williams G M
J Immunol Methods. 1977;17(3-4):225-39. doi: 10.1016/0022-1759(77)90105-3.
Four separate assays of human antibody reactivity to four separate normal and malignant human tissue culture cell lines from two patients have been evaluated using a single highly-reactive allogeneic serum. The visual and end-point cytolysis assay and the 51Chromium release assay were equally sensitive in measuring complement mediated antibody cytoxicity and both were far more sensitive than a trypan blue dye exclusion assay. The assay of antibody reactivity by hemadsorption technique was about 10 times more sensitive than any of the cytotoxicity assays. This latter assay measures only IgG antibody however. These assays showed that cell lines from different patients may differ greatly in 'reactivity' to an allogeneic serum and emphasized the importance of utilizing tumor and normal cells from the same patient when using tissue culture cells to search for tumor specific reactivity. These observations emphasize the importance of utilizing multiple assays against paired normal and malignant cells from the same patient to be certain of the specificity and magnitude of the measured antibody.
使用一种高度反应性的同种异体血清，对来自两名患者的四种不同的正常和恶性人组织培养细胞系的人抗体反应性进行了四项独立检测。视觉和终点细胞溶解试验以及51铬释放试验在测量补体介导的抗体细胞毒性方面同样敏感，并且两者都比台盼蓝染料排除试验敏感得多。通过血细胞吸附技术进行的抗体反应性检测比任何一种细胞毒性检测灵敏约10倍。然而，后一种检测仅测量IgG抗体。这些检测表明，来自不同患者的细胞系对同种异体血清的“反应性”可能有很大差异，并强调了在使用组织培养细胞寻找肿瘤特异性反应性时，利用来自同一患者的肿瘤细胞和正常细胞的重要性。这些观察结果强调了对来自同一患者的配对正常细胞和恶性细胞进行多种检测以确定所测抗体的特异性和强度的重要性。
