Le Lann Klervi, Kraffe Edouard, Kervarec Nelly, Cerantola Stéphane, Payri Claude Elisabeth, Stiger-Pouvreau Valérie
LEMAR UMR 6539 UBO CNRS IRD, European Institute of Marine Studies (IUEM), University of Western Brittany (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, Plouzané, 29280, France.
RMN-RPE-SM, 6 avenue, Victor-Le-Gorgeu-CS93837, Brest Cedex 3, 29238, France.
J Phycol. 2014 Dec;50(6):1048-57. doi: 10.1111/jpy.12235. Epub 2014 Oct 16.
Several species of the genus Turbinaria coexist along the coasts of islands in the Indian and Pacific Oceans. Among these brown algae, Turbinaria ornata and T. conoides are sister species that are difficult to differentiate using exclusively morphological characters. Based on in vivo nuclear magnetic resonance and chromatographic techniques, i.e., liquid and gas chromatography-mass spectrometry analysis, combined with phylogenetic data, we successfully identified turbinaric acid in T. conoides samples from several Indian and Pacific Ocean islands. This nonvariable discriminant molecule was only identified in T. conoides specimens, but not in the two allied species T. ornata and T. decurrens. Results are discussed with regard to turbinaric acid as an interesting chemomarker isolated from T. conoides and the rapid discrimination of Turbinaria specimens using chemical assays.
旋花藻属的几个物种共存于印度洋和太平洋岛屿的海岸沿线。在这些褐藻中，华丽旋花藻和锥状旋花藻是姊妹物种，仅使用形态特征很难区分它们。基于体内核磁共振和色谱技术，即液相和气相色谱 - 质谱分析，并结合系统发育数据，我们成功地在来自印度洋和太平洋多个岛屿的锥状旋花藻样本中鉴定出了旋花藻酸。这种不变的判别分子仅在锥状旋花藻标本中被鉴定出来，而在两个近缘物种华丽旋花藻和下延旋花藻中未被鉴定到。本文讨论了旋花藻酸作为一种从锥状旋花藻中分离出的有趣化学标志物以及使用化学分析快速鉴别旋花藻标本的相关结果。
