Andreeva N S, Gushchina A E, Zhdanov A S, Pechik I V, Safro M G, Fedorov A A
Mol Biol (Mosk). 1989 Nov-Dec;23(6):1523-34.
The paper is a brief account of aspartic proteinases' structural studies developed in V.A. Engelhardt Institute of Molecular Biology during the last 3 years. The work on porcine pepsin has been finalized after the refinement of the monoclinic crystal form at 1.8 A resolution performed in collaboration with the group of protein structure and function studies of the University of Alberta in Canada. An important structural property of chymosin which explains the enzyme specificity has been found. Protein engineering work on chymosin is being developed. The structural template for aspartic proteinases has been elucidated and on the basis of this template the model of HIV-1 protease molecule has been built. Some approaches to the design of HIV-1 protease inhibitors were elucidated.
本文简要介绍了过去三年在俄罗斯科学院恩格尔哈特分子生物学研究所开展的天冬氨酸蛋白酶结构研究。与加拿大阿尔伯塔大学蛋白质结构与功能研究小组合作，在1.8埃分辨率下对猪胃蛋白酶单斜晶型进行优化后，猪胃蛋白酶的研究工作已完成。已发现凝乳酶的一个重要结构特性，该特性解释了酶的特异性。凝乳酶的蛋白质工程工作正在开展。已阐明天冬氨酸蛋白酶的结构模板，并在此模板基础上构建了HIV-1蛋白酶分子模型。阐明了一些设计HIV-1蛋白酶抑制剂的方法。
