微生物辅助高抗冲聚苯乙烯（HIPS）降解。
Microbial assisted High Impact Polystyrene (HIPS) degradation.
机构信息
Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India.
Bio-Fuels Division (BFD), CSIR-Indian Institute of Petroleum (IIP), Dehradun, Uttarakhand 248005, India.
出版信息
Bioresour Technol. 2016 Aug;213:204-207. doi: 10.1016/j.biortech.2016.03.021. Epub 2016 Mar 8.
The efficacy of newly isolated Pseudomonas and Bacillus strains to degrade brominated High Impact Polystyrene (HIPS) was investigated. Viability of these cultures while using e-plastic as sole carbon source was validated through Triphenyl Tetrazolium Chloride (TTC). Four days incubation of HIPS emulsion with Bacillus spp. showed 94% reduction in turbidity and was 97% with Pseudomonas spp. Confirmation of degradation was concluded by HPLC, NMR, FTIR, TGA and weight loss analysis. NMR spectra of the degraded film revealed the formation of aliphatic carbon chain with bromine and its release. FTIR analysis of the samples showed a reduction in CH, CO and CN groups. Surface changes in the brominated HIPS film was visualized through SEM analysis. Degradation with Bacillus spp showed a weight loss of 23% (w/w) of HIPS film in 30days.
研究了新分离的假单胞菌和芽孢杆菌菌株对降解溴化高冲击聚苯乙烯（HIPS）的功效。通过三苯基四氮唑氯化物（TTC）验证了这些培养物在使用电子塑料作为唯一碳源时的生存能力。用芽孢杆菌属菌株孵育 HIPS 乳液四天，浊度降低了 94%，用假单胞菌属菌株降低了 97%。通过 HPLC、NMR、FTIR、TGA 和失重分析得出了降解的结论。降解膜的 NMR 谱表明形成了带有溴的脂肪族碳链及其释放。样品的 FTIR 分析表明 CH、CO 和 CN 基团减少。通过 SEM 分析观察到溴化 HIPS 薄膜的表面变化。芽孢杆菌属菌株的降解在 30 天内使 HIPS 薄膜的重量损失了 23%（w/w）。
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