镉（+2价）、铬（+6价）、铜（+2价）对博氏半线脂鲤（鱼类，无齿脂鲤科）鳃和肝脏的遗传毒性效应
Genotoxic Effects Induced by Cd(+2), Cr(+6), Cu(+2) in the Gill and Liver of Odontesthes bonariensis (Piscies, Atherinopsidae).
作者信息
Gasulla J, Picco S J, Carriquiriborde P, Dulout F N, Ronco A E, de Luca J C
机构信息
Instituto de Microbiología y Zoología Agrícola (IMyZA), Instituto Nacional de Tecnología Agropecuaria (INTA) - CONICET, Las Cabañas y De los Reseros s/n, Hurlingham, Buenos Aires, Argentina.
Instituto de Genética Veterinaria "Fernando Noel Dulout" (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, Calle 60 y 117 (1900), La Plata, Buenos Aires, Argentina.
出版信息
Bull Environ Contam Toxicol. 2016 May;96(5):591-5. doi: 10.1007/s00128-016-1774-y. Epub 2016 Mar 22.
Genotoxic effects of Cd(+2), Cr(+6), and Cu(+2) on the gill and liver of the Argentinean Silverside (Odontesthes bonariensis) were studied using the comet assay and in relation with the metal tissue accumulation. Fish were exposed to three waterborne concentrations of each metal for 2 and 16 days. Genotoxicity was assessed by the single cell gel electrophoresis (comet assay). After 2 days, significant increase of the genetic damage index (GDI) was only observed in the gill of fish exposed to Cr(+6) and Cu(+2), and the LOECs were 2160 nM and 921.1 nM, respectively. The gill LOEC for Cd(+2) by 16 days was 9.4 nM. In the liver, LOECs were obtained only for Cd(+2) and Cr(+6) and were 9.4 and 2160 nM, respectively. The three metals were able to induce genotoxic effects at environmentally relevant concentrations and the gill was the most sensitive organ.
采用彗星试验研究了镉（+2）、铬（+6）和铜（+2）对阿根廷银汉鱼（Odontesthes bonariensis）鳃和肝脏的遗传毒性作用，并研究了其与金属组织蓄积的关系。将鱼暴露于每种金属的三种水体浓度下2天和16天。通过单细胞凝胶电泳（彗星试验）评估遗传毒性。2天后，仅在暴露于铬（+6）和铜（+2）的鱼的鳃中观察到遗传损伤指数（GDI）显著增加，最低观察效应浓度（LOEC）分别为2160 nM和921.1 nM。镉（+2）暴露16天时鳃的LOEC为9.4 nM。在肝脏中，仅获得了镉（+2）和铬（+6）的LOEC，分别为9.4 nM和2160 nM。这三种金属在环境相关浓度下均能诱导遗传毒性作用，鳃是最敏感的器官。
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