希茉莉提取物的抗氧化和保肝活性。

Antioxidant and hepatoprotective activity of Hamelia patens extracts.

作者信息

Perez-Meseguer Jonathan, Delgado-Montemayor Cecilia, Ortíz-Torres Tania, Salazar-Aranda Ricardo, Cordero-Perez Paula, de Torres Noemí Waksman

机构信息

Universidad Autónoma de Nuevo León, Departamento de Química Analítica, Facultad de Medicina. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro. CP 64460. Monterrey, NL. México.

Universidad Autónoma de Nuevo León, Unidad de Hígado. Facultad de Medicina y Hospital Universitario "Dr. José Eleuterio González. Av. Fancisco I. Madero y Dr Aguirre Pequeño S/N. Col Mitras Centro, CP 64460. Monterrey, NL. México.

出版信息

Pak J Pharm Sci. 2016 Jan;29(1 Suppl):343-8.

PMID:27005511

Abstract

Hamelia patens is widely used in the traditional medicine of Mexico and Central America for the treatment of illnesses associated with inflammatory processes. In this study, antioxidant and hepatoprotective activity were assayed on the methanolic crude (ME), hexane (HE), ethyl acetate (AE), and butanol (BE) extracts of H. patens. The total phenolic content (TPC) as mg of gallic acid equivalents per g of dry extract was determined by Folin-Ciocalteu's method (ME=141.58±11.99, HE=33.96±1.13, AE=375.18±13.09, BE=132.08±3.62), and antioxidant activity by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical-scavenging method (EC(50) ME=77.87±5.67, HE=236.64±26.32, AE=45.87±2.24, BE=50.97±0.85μg/mL). Hepatoprotective activity was evaluated through AST activity on HepG2 cells subjected to damage with CCl(4) (ME=62.5±3.41, HE=72.25±2.87, AE=63.50±4.20, BE=43.74±4.03). BE showed the greater hepatoprotective activity and a good antioxidant capacity, while HE did not show hepatoprotective or antioxidant activity. Cytotoxicity was evaluated on Vero cells cultures; none showed significant toxicity.

摘要

希茉莉在墨西哥和中美洲的传统医学中被广泛用于治疗与炎症过程相关的疾病。在本研究中，对希茉莉的甲醇粗提物（ME）、己烷提取物（HE）、乙酸乙酯提取物（AE）和丁醇提取物（BE）进行了抗氧化和保肝活性测定。通过福林-酚试剂法测定每克干提取物中没食子酸当量的毫克数来确定总酚含量（TPC）（ME = 141.58±11.99，HE = 33.96±1.13，AE = 375.18±13.09，BE = 132.08±3.62），并通过2,2-二苯基-1-苦基肼基（DPPH）自由基清除法测定抗氧化活性（EC50 ME = 77.87±5.67，HE = 236.64±26.32，AE = 45.87±2.24，BE = 50.97±0.85μg/mL）。通过对经四氯化碳损伤的HepG2细胞的天冬氨酸转氨酶（AST）活性评估保肝活性（ME = 62.5±3.41，HE = 72.25±2.87，AE = 63.50±4.20，BE = 43.74±4.03）。BE显示出更强的保肝活性和良好的抗氧化能力，而HE未显示出保肝或抗氧化活性。在Vero细胞培养物上评估细胞毒性；均未显示出明显毒性。