National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Food Chem. 2016 Aug 15;205:272-9. doi: 10.1016/j.foodchem.2016.02.157. Epub 2016 Mar 3.
Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities.
通过全基因组序列分析，鉴定出一种未知的转基因木瓜（Carica papaya L.）。利用实时聚合酶链反应（PCR）监测检测到一种转基因阳性新鲜木瓜果实商品，为其生成了全基因组序列数据。获得的序列与木瓜基因组序列的开放数据库进行了比对。鉴定出的转基因构建体和事件特异性序列是为了抵抗木瓜环斑病毒感染而开发的转基因木瓜。基于转基因序列，开发了一种适用于各种食品的转基因木瓜的特定实时 PCR 检测方法。全基因组序列分析能够鉴定转基因木瓜中的未知转基因构建体和事件特异性序列，并开发出一种在木瓜食品中检测它们的可靠方法。
