Bellagamba Bruno Corrêa, Abreu Bianca Regina Ribas de, Grivicich Ivana, Markarian Carolina Franke, Chem Eduardo, Camassola Melissa, Nardi Nance Beyer, Dihl Rafael Rodrigues
Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Universidade Luterana do Brasil, Canoas, RS, Brazil.
Genet Mol Biol. 2016 Mar;39(1):129-34. doi: 10.1590/1678-4685-GMB-2015-0057.
Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS, which was not observed when OVCAR-3 cells were exposed to this drug.
间充质干细胞（MSCs）以其重要特性而闻名，这些特性包括多谱系分化潜能、营养因子分泌以及在各种器官和组织中的定位。然而，间充质干细胞在肿瘤微环境中也扮演着特殊角色，它们可分化为肿瘤相关成纤维细胞，或通过不同机制支持肿瘤生长。顺铂（CIS）是一种广泛应用于多种癌症治疗的药物，在体外和体内均具有细胞毒性和基因毒性。在此，我们通过MTT和彗星试验评估了顺铂对间充质干细胞和卵巢癌细胞系OVCAR-3的影响。我们的结果表明，间充质干细胞对顺铂诱导的细胞死亡和DNA损伤具有抗性，而当OVCAR-3细胞暴露于这种药物时则未观察到这种抗性。
