Rinkel Barbara E, Hayes Paul, Gueidan Cécile, Brodie Juliet
Department of Botany, Natural History Museum, Cromwell Road, London SW7 5BD, UKFaculty of Science, University of Portsmouth, St Michael's Building, White Swan Road, Portsmouth PO1 2DT, UKDepartment of Botany, Natural History Museum, Cromwell Road, London SW7 5BD, UK.
J Phycol. 2012 Aug;48(4):1020-7. doi: 10.1111/j.1529-8817.2012.01196.x. Epub 2012 Jun 28.
A molecular phylogeny was reconstructed from a culture collection of >150 isolates of epi-endophytic and endophytic green algae, based on nucleotide sequences of the plastid tufA and nuclear ITS2 loci. The cultures were isolated from a variety of algal hosts, notably the red algae Chondrus crispus, Mastocarpus stellatus, and Osmundea species, and the brown algae Chorda filum and Fucus serratus. The phylogeny revealed that in the Ulvales the majority of isolates fell into Acrochaete (Ulvellaceae), Ulva (Ulvaceae), Bolbocoleon (Bolbocoleaceae), and at least two unknown genera provisionally assigned to the Kornmanniaceae. Acrochaete was monophyletic. The genus was also more specious than previously described with 12 species, including up to six new species awaiting formal description. Isolates identified as Acrochaete repens, the type species of the genus, were polyphyletic. The remainder of the isolates were placed in the Ulotrichales. The results confirm that the endophytic habit supports a broad diversity of algal taxa and suggest that blade formation is a relatively recent innovation within the green algae.
基于质体tufA和核ITS2基因座的核苷酸序列，从150多个表生内生和内生绿藻分离株的培养物中重建了分子系统发育。这些培养物是从多种藻类宿主中分离出来的，特别是红藻皱波角叉菜、星状鹿角菜和奥斯芒藻属物种，以及褐藻绳藻和锯齿墨角藻。系统发育显示，在石莼目中，大多数分离株属于顶毛藻属（石莼科）、石莼属（石莼科）、球囊藻属（球囊藻科），以及至少两个暂时归入科尔曼藻科的未知属。顶毛藻属是单系的。该属也比以前描述的更为多样，有12个物种，包括多达6个有待正式描述的新物种。被鉴定为该属模式种的匍匐顶毛藻的分离株是多系的。其余分离株被归入丝藻目。结果证实内生习性支持藻类分类群的广泛多样性，并表明叶片形成是绿藻中相对较新的创新。
