Tiwari Aseem K, Pandey Prashant, Subbaraman Harini, Bhargava Rahul, Rawat Ganesh, Madiraju Shivani, Raina Vimarsh, Bhargava Richa
Department of Transfusion Medicine, Medanta-The Medicity, Sector-38, Gurgaon, Haryana, India.
Department of Transfusion Medicine, Jaypee Hospital, Sector-128, Noida, Uttar Pradesh, India.
Asian J Transfus Sci. 2016 Jan-Jun;10(1):93-7. doi: 10.4103/0973-6247.164273.
Harvest of hematopoietic progenitor cells via leukapheresis is being used increasingly for transplants in India. Adequate yield of cells per kilogram body weight of recipient is required for successful engraftment. Collection efficiency (CE) is an objective quality parameter used to assess the quality of leukapheresis program. In this study, we calculated the CE of the ComTec cell separator (Fresenius Kabi, Germany) using two different formulae (CE1 and CE2) and analyzed various patient and procedural factors, which may affect it.
One hundred and one consecutive procedures in 77 autologous donors carried out over 3 years period were retrospectively reviewed. Various characteristics like gender, age, weight, disease status, hematocrit, preprocedure total leukocyte count, preprocedure CD34 positive (CD34+) cells count, preprocedure absolute CD34+ cell count and processed apheresis volume effect on CE were compared. CE for each procedure was calculated using two different formulae, and results were compared using statistical correlation and regression analysis.
The mean CE1 and CE2 was 41.2 and 49.1, respectively. CE2 appeared to be more accurate indicator of overall CE as it considered the impact of continued mobilization of stem cells during apheresis procedure, itself. Of all the factors affecting CE, preprocedure absolute CD34+ was the only independent factor affecting CE.
The only factor affecting CE was preprocedure absolute CD34+ cells. Though the mean CE2 was higher than CE1, it was not statistically significant.
在印度，通过白细胞单采术采集造血祖细胞越来越多地用于移植。为实现成功植入，每千克受体体重需要有足够的细胞产量。采集效率（CE）是用于评估白细胞单采术质量的一个客观质量参数。在本研究中，我们使用两种不同公式（CE1和CE2）计算了ComTec细胞分离器（德国费森尤斯卡比公司）的采集效率，并分析了可能影响它的各种患者和操作因素。
回顾性分析了77例自体供者在3年期间进行的101次连续操作。比较了性别、年龄、体重、疾病状态、血细胞比容、术前总白细胞计数、术前CD34阳性（CD34+）细胞计数、术前绝对CD34+细胞计数以及处理的单采血量对采集效率的影响。使用两种不同公式计算每次操作的采集效率，并通过统计相关性和回归分析比较结果。
CE1和CE2的平均值分别为41.2和49.1。CE2似乎是总体采集效率更准确的指标，因为它本身考虑了单采过程中干细胞持续动员的影响。在所有影响采集效率的因素中，术前绝对CD34+是影响采集效率的唯一独立因素。
影响采集效率的唯一因素是术前绝对CD34+细胞。虽然CE2的平均值高于CE1，但差异无统计学意义。
