Fazeli Zahra, Ghaderian Sayyed Mohammad Hossein, Rajabibazl Masoumeh, Salami Siamak, Vazifeh Shiran Nader, Omrani Mir Davood
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Shahid Labbafi Nejad Educational Hospital, Tehran, Iran.
Int J Mol Cell Med. 2015 Fall;4(4):209-17.
Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, β III tubulin, NFM, MAP2 and NSE) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of β-tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine.
间充质干细胞（MSCs）在适当的培养条件下具有分化为神经元样细胞的能力。在本研究中，我们调查了源自人外周血的间充质干细胞（PB-MSCs）是否能通过生长因子表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）和头蛋白（Noggin）的协同作用分化为神经元样细胞。为此，通过SYBR Green实时定量PCR评估经处理的PB-MSCs中五种神经元标志物（巢蛋白、β III微管蛋白、神经丝蛋白（NFM）、微管相关蛋白2（MAP2）和神经元特异性烯醇化酶（NSE））的表达。表达分析表明，与作为对照组的未处理PB-MSCs相比，经处理的PB-MSCs中β-微管蛋白和NFM的表达更高。在用Noggin处理的PB-MSCs中，巢蛋白的表达也有所减少。本研究表明，用Noggin联合bFGF和EGF处理PB-MSCs可能会使这些细胞分化为神经谱系细胞。所获得的结果可在再生医学的有益应用中进一步发展。
