Department of Desalination and Water Treatment, Zuckerberg Institute for Water Research, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus, 8499000, Israel.
Technische Universität München, Forschungs-Neutronenquelle Heinz Maier-Leibnitz (FRM II), D-85748 Garching, Germany.
Colloids Surf B Biointerfaces. 2016 Jul 1;143:233-242. doi: 10.1016/j.colsurfb.2016.02.047. Epub 2016 Feb 26.
Biofouling and scaling on reverse osmosis (RO) or nanofiltration (NF) membranes during desalination of secondary and tertiary effluents pose an obstacle that limits the reuse of wastewater. In this study we explored the mineral scaling induced by biopolymers originated from bacterial biofilms: bovine serum albumin (BSA), fibrinogen, lysozyme and alginic acid, as well as an extracts of extracellular polymeric substances (EPS) from bio-fouled RO membranes from wastewater treatment facility. Mineralization studies were performed on Langmuir films of the biopolymers deposited at the interface of a solution simulating RO desalination of secondary-treated wastewater effluents. All studied biopolymers and EPS induced heterogeneous mineralization of mainly calcium phosphate. Using IR spectroscopy coupled with systematic quantitative analysis of the surface pressure versus molecular-area isotherms, we determined the mineralization tendencies of the biopolymers to be in the order of: fibrinogen>lysozyme>BSA>alginic acid. The biopolymers and EPS studied here were found to be accelerators of calcium-phosphate mineralization. This study demonstrates the utilization of Langmuir surface-pressure area isotherms and a model solution in quantitatively assessing the mineralization tendencies of various molecular components of EPS in context of membrane-based water treatment systems.
在二级和三级废水的脱盐过程中，反渗透 (RO) 或纳滤 (NF) 膜上的生物污垢和结垢是一个限制废水再利用的障碍。在这项研究中，我们探索了源自细菌生物膜的生物聚合物引起的矿物结垢：牛血清白蛋白 (BSA)、纤维蛋白原、溶菌酶和藻酸，以及从废水处理设施的 RO 膜生物污垢中提取的细胞外聚合物物质 (EPS)。在模拟二级处理废水 RO 脱盐的溶液界面上沉积的生物聚合物的 Langmuir 膜上进行了矿化研究。所有研究的生物聚合物和 EPS 都诱导了主要为磷酸钙的非均相矿化。通过与表面压力与分子面积等温线的系统定量分析相结合的红外光谱，我们确定生物聚合物的矿化趋势顺序为：纤维蛋白原>溶菌酶>BSA>藻酸。研究中发现，这些生物聚合物和 EPS 是促进磷酸钙矿化的加速剂。这项研究证明了在膜基水处理系统中，利用 Langmuir 表面压力-面积等温线和模型溶液定量评估 EPS 各种分子成分的矿化趋势的方法。
