Li Yang, Tang Wenzhao, Chen Jing, Jia Ru, Ma Lianjie, Wang Shaoli, Wang Jiao, Shen Xiangling, Chu Zhaohui, Zhu Changxiang, Ding Xinhua
State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University , Taian 271018, Shandong, P. R. China.
Key Laboratory for Rare Disease of Shandong Province, Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062, Shandong, P. R. China.
J Agric Food Chem. 2016 Apr 13;64(14):2932-40. doi: 10.1021/acs.jafc.6b00270. Epub 2016 Apr 4.
Potato (Solanum tuberosum L.) is a major crop worldwide that meets human economic and nutritional requirements. Potato has several advantages over other crops: easy to cultivate and store, cheap to consume, and rich in a variety of secondary metabolites. In this study, we generated three marker-free transgenic potato lines that expressed the Arabidopsis thaliana flavonol-specific transcriptional activator AtMYB12 driven by the tuber-specific promoter Patatin. Marker-free potato tubers displayed increased amounts of caffeoylquinic acids (CQAs) (3.35-fold increases on average) and flavonols (4.50-fold increase on average). Concentrations of these metabolites were associated with the enhanced expression of genes in the CQA and flavonol biosynthesis pathways. Accumulation of CQAs and flavonols resulted in 2-fold higher antioxidant capacity compared to wild-type potatoes. Tubers from these marker-free transgenic potatoes have therefore improved antioxidant properties.
马铃薯（Solanum tuberosum L.）是一种满足人类经济和营养需求的全球主要作物。与其他作物相比，马铃薯具有几个优势：易于种植和储存、消费成本低，并且富含多种次生代谢产物。在本研究中，我们培育了三个无标记转基因马铃薯品系，这些品系表达了由块茎特异性启动子Patatin驱动的拟南芥黄酮醇特异性转录激活因子AtMYB12。无标记马铃薯块茎中咖啡酰奎宁酸（CQAs）的含量增加（平均增加3.35倍），黄酮醇含量也增加（平均增加4.50倍）。这些代谢产物的浓度与CQAs和黄酮醇生物合成途径中基因的表达增强有关。与野生型马铃薯相比，CQAs和黄酮醇的积累使抗氧化能力提高了2倍。因此，这些无标记转基因马铃薯的块茎具有改善的抗氧化特性。
