Gerecht Andrea, Romano Giovanna, Ianora Adrianna, d'Ippolito Giuliana, Cutignano Adele, Fontana Angelo
Functional and Evolutionary Ecology Laboratory, Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Naples, ItalyCNR-Institute Biomolecular Chemistry, Via Campi Flegrei 34, 80078 Pozzuoli (Naples), Italy.
J Phycol. 2011 Oct;47(5):1050-6. doi: 10.1111/j.1529-8817.2011.01030.x. Epub 2011 Jul 21.
Diatom oxylipins have been observed to deleteriously impact copepod reproductive success. However, field studies have revealed very variable and case-dependent results. Therefore, the plasticity of diatom oxylipin metabolism was studied among four clones of the marine diatom Skeletonema marinoi Sarno et Zingone. Diatom oxylipin metabolism was studied by two lipoxygenase (LOX) activity assays carried out at different pH values and by oxylipin quantification. The four clones showed no major metabolic differences in terms of protein content or growth rate. However, two of the clones produced significantly higher levels of oxylipins than the other two. LOX activity measurements also indicated clonal variability in fatty acid oxidative metabolism. The presence of clone-specific differences in oxylipin metabolism may play a role in shaping diatom population dynamics by conferring selective advantages to certain clones.
已观察到硅藻氧脂对桡足类动物的繁殖成功率产生有害影响。然而，实地研究显示结果差异很大且因情况而异。因此，对海洋硅藻玛氏骨条藻（Skeletonema marinoi Sarno et Zingone）的四个克隆株进行了硅藻氧脂代谢可塑性的研究。通过在不同pH值下进行的两种脂氧合酶（LOX）活性测定以及氧脂定量研究了硅藻氧脂代谢。这四个克隆株在蛋白质含量或生长速率方面没有显著的代谢差异。然而，其中两个克隆株产生的氧脂水平明显高于另外两个。LOX活性测量也表明脂肪酸氧化代谢存在克隆变异性。氧脂代谢中克隆特异性差异的存在可能通过赋予某些克隆株选择性优势而在塑造硅藻种群动态中发挥作用。
