Ross Daniela, Altmann Michael
Institute of Biochemistry and Molecular Medicine (IBMM), Bühlstrasse 28, 3012 Bern, Switzerland.
Methods. 2016 Sep 1;107:57-62. doi: 10.1016/j.ymeth.2016.03.021. Epub 2016 Mar 25.
We present a protocol that enables easy extraction of translating mRNAs by purification of tagged ribosomes and associated mRNAs. By studying three different examples of stress influenced genes, we demonstrate the effectiveness and compare our rapid method to an older protocol (Halbeisen et al., 2009).
我们提出了一种方案，该方案能够通过纯化带标签的核糖体及相关mRNA来轻松提取正在翻译的mRNA。通过研究应激影响基因的三个不同实例，我们证明了该方法的有效性，并将我们的快速方法与一种较旧的方案（Halbeisen等人，2009年）进行了比较。
