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[免疫酶分析采用化学发光和比色检测法检测禽流感病毒和新城疫抗原及抗体的敏感性和特异性比较研究]

[Comparative research into sensitivity and specificity of immune-enzyme analysis with chemiluminescence and colorimetric detection for detecting antigens and antibodies to avian influenza viruses and newcastle disease].

作者信息

Vitkova O N, Kapustina T P, Mikhailova V V, Safonov G A, Vlasova N N, Belousova R V

出版信息

Vopr Virusol. 2015;60(6):41-5.

Abstract

The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno- chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.

摘要

这项工作的目标是展示用于检测禽流感和新城疫病毒特定病毒抗原及抗体的化学发光检测和比色检测的酶联免疫吸附试验的研发成果

免疫化学发光测定法具有高灵敏度和特异性,比酶联免疫吸附试验比色法高10至50倍。结果的高效性以及实验室检测过程的自动化(使用发光计)使得这些方法可被推荐用于这些传染病的初步筛查试验。

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