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[沙眼衣原体泌尿生殖系统感染的一些诊断方面]

[SOME DIAGNOSTIC ASPECTS OF CHLAMYDIA TRACHOMATIS UROGENITAL INFECTIONS].

作者信息

Kovachev E, Bakardzhiev I, Pehlivanov G, Kolev N, Grueva E, Anzhel S

出版信息

Akush Ginekol (Sofiia). 2015;54(8):8-12.

Abstract

The following tests were used to make the diagnosis of Chlamydia Trachomatis: Ensime Immunoassay (EIA), Enzyme-Linked FluorescentAssay (ELFA), Polymerase Chain Reaction DNA amplification for Chlamydia Trachomatis, Neisseria Gonorrhoeae, Mycoplasma Genitalium and Ureaplasma Urealyticum. The subject of this study were 486 outpatients between the ages of 17 and 62, diagnosed with urethritis, epidydimitis, prostatitis, cervicitis, endocervicitis, pelvic inflammatory disease and sterility. Comparison PCR and EIA showed statistically significant difference between the positive results obtained by the two methods (p<0.001). Comparing PCR and ELFA on the other hand, did not show any statistically significant difference (p>0.1). EIA is the method that gives a higher percentage of nonspecific positive reactions, while the results obtained with ELFA are much closer to the ones obtained by PCR. According to contemporary evidence based medicine, the development of unified standart methods for diagnosis and evaluation is of vital importance, not only for the patient and the treating physician but also concerns for the healthcare system, the general public and the pharmaceutical companies as well.

摘要

以下检测方法用于诊断沙眼衣原体

酶免疫测定法(EIA)、酶联荧光免疫测定法(ELFA)、针对沙眼衣原体、淋病奈瑟菌、生殖支原体和解脲脲原体的聚合酶链反应DNA扩增法。本研究的对象为486名年龄在17至62岁之间的门诊患者,他们被诊断患有尿道炎、附睾炎、前列腺炎、宫颈炎、子宫内膜炎、盆腔炎和不育症。比较PCR和EIA发现,两种方法获得的阳性结果之间存在统计学显著差异(p<0.001)。另一方面,比较PCR和ELFA则未显示出任何统计学显著差异(p>0.1)。EIA是产生非特异性阳性反应百分比更高的方法,而ELFA获得的结果与PCR获得的结果更为接近。根据当代循证医学,开发统一的诊断和评估标准方法至关重要,这不仅对患者和治疗医生而言,也关乎医疗保健系统、公众和制药公司。

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