评估一种通过Cobas Amplicor聚合酶链反应从不同样本材料中检测沙眼衣原体和淋病奈瑟菌的自动提取方法。
Evaluation of an automated extraction method for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Cobas Amplicor PCR from different sample materials.
作者信息
Geertsen Ralf, Friderich Paul, Dobec Marinko, Emler Stefan
机构信息
Institute Virion Ltd., Molecular Biology, Rueschlikon, Switzerland.
出版信息
Scand J Infect Dis. 2007;39(5):405-8. doi: 10.1080/00365540601087582.
PMID:17464862
Abstract
A commercially available automated device (MagNA Pure LC) was adapted for nucleic acid extraction of urogenital specimen for subsequent PCR detection of Chlamydia trachomatis and Neisseria gonorrhoeae in a clinical laboratory. Results were compared to the standard manual extraction procedure and showed excellent correlation, with even slightly increased sensitivity.
摘要
在一家临床实验室中，对一种市售的自动化设备（MagNA Pure LC）进行了改造，用于泌尿生殖标本的核酸提取，以便随后对沙眼衣原体和淋病奈瑟菌进行PCR检测。将结果与标准手动提取程序进行比较，结果显示具有极好的相关性，甚至灵敏度略有提高。
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