氚标记米索硝唑在小鼠体内的放射自显影研究。

Autoradiographic study of tritium-labeled misonidazole in the mouse.

作者信息

Cobb L M, Nolan J

机构信息

Division of Experimental Pathology and Therapeutics, MRC Radiobiology Unit, Didcot, Oxon, United Kingdom.

出版信息

Int J Radiat Oncol Biol Phys. 1989 Apr;16(4):953-6. doi: 10.1016/0360-3016(89)90894-8.

DOI:10.1016/0360-3016(89)90894-8

PMID:2703403

Abstract

The localization of tritiated misonidazole metabolites in a number of normal tissues in the mouse is reported from autoradiography. The labeled misonidazole was injected at 750 or 75 mg/kg body weight (Rel. Sp. Act. 74 and 740 MBq/mg respectively). The grain count ratio, parenchyma:stroma, for selected tissues was: liver (centrilobular zone) 13; meibomian gland (acini) 68, (duct) 116; esophagus (keratinized layer) 61; enamel organ 17. It is concluded that there are a number of tissues which will accumulate MISO metabolites although they may not all be hypoxic.

摘要

通过放射自显影术报告了氚标记的米索硝唑代谢产物在小鼠多种正常组织中的定位情况。分别以750或75mg/kg体重注射标记的米索硝唑（比活度分别为74和740MBq/mg）。选定组织的实质：间质颗粒计数比为：肝脏（小叶中心区）13；睑板腺（腺泡）68，（导管）116；食管（角质化层）61；牙釉质器官17。得出的结论是，有许多组织会积累米索硝唑代谢产物，尽管它们可能并非全部处于缺氧状态。