Falls Theresa, Roy Dominic Guy, Bell John Cameron, Bourgeois-Daigneault Marie-Claude
Theresa Falls is a research technician at the Centre for Innovative Cancer Research at Ottawa Hospital Research Institute in Ottawa, Canada. Dominic Guy Roy is a Ph.D candidate at the Centre for Innovative Cancer Research at Ottawa Hospital Research Institute in Ottawa, Canada, and a Ph.D candidate in the Biochemistry, Microbiology, and Immunology Department at the University of Ottawa in Ottawa, Canada. John Cameron Bell is a senior researcher at the Centre for Innovative Cancer Research at Ottawa Hospital Research Institute in Ottawa, Canada, and professor in the Biochemistry, Microbiology, and Immunology Department at the University of Ottawa in Ottawa, Canada. Marie-Claude Bourgeois-Daigneault is a postdoctoral fellow at the Centre for Innovative Cancer Research at Ottawa Hospital Research Institute in Ottawa, Canada, and a postdoctoral fellow in the Biochemistry, Microbiology, and Immunology Department at the University of Ottawa in Ottawa, Canada.
ILAR J. 2016;57(1):73-85. doi: 10.1093/ilar/ilv048.
The preclinical optimization and validation of novel treatments for cancer therapy requires the use of laboratory animals. Although in vitro experiments using tumor cell lines and ex vivo treatment of patient tumor samples provide a remarkable first-line tool for the initial study of tumoricidal potential, tumor-bearing animals remain the primary option to study delivery, efficacy, and safety of therapies in the context of a complete tumor microenvironment and functional immune system. In this review, we will describe the use of murine tumor models for oncolytic virotherapy using vesicular stomatitis virus. We will discuss studies using immunocompetent and immunodeficient models with respect to toxicity and therapeutic treatments, as well as the various techniques and tools available to study cancer therapy with Rhabdoviruses.
癌症治疗新疗法的临床前优化和验证需要使用实验动物。尽管使用肿瘤细胞系的体外实验和患者肿瘤样本的离体治疗为初步研究杀肿瘤潜力提供了出色的一线工具，但荷瘤动物仍然是在完整肿瘤微环境和功能性免疫系统背景下研究治疗方法的递送、疗效和安全性的主要选择。在这篇综述中，我们将描述使用水泡性口炎病毒进行溶瘤病毒疗法的小鼠肿瘤模型的应用。我们将讨论使用免疫健全和免疫缺陷模型进行毒性和治疗性治疗的研究，以及可用于研究弹状病毒癌症治疗的各种技术和工具。
