Sauer W C, den Hartog L A, Huisman J, van Leeuwen P, de Lange C F
Agricultural University of The Netherlands, Wageningen.
J Anim Sci. 1989 Feb;67(2):432-40. doi: 10.2527/jas1989.672432x.
Studies were conducted with four (50 to 70 kg) barrows to evaluate the mobile nylon bag technique (MNBT) for determining the apparent protein digestibility (AD) in a variety of feedstuffs. One-gram samples of each feedstuff, ground through a 1.0-mm mesh screen, were enclosed in nylon bags (25 X 40 mm; 48 microns mesh). Following predigestion (.01 N HCl; 4,000 Folin Intestinal Pepsin Units/liter), four bags of each sample were inserted into each pig via a duodenal T-cannula. The indigested contents were pooled within pig and feedstuff prior to crude protein analysis. The AD of each feedstuff was determined previously with four barrows according to the conventional method (CM), based on a 5-d total collection of feces following a 14-d adaptation period. The following results, AD (%), were obtained with the MNBT (n = 4) and CM (n = 4), respectively. Barley, 70.1 vs 81.2 (P less than .05); corn, 72.3 vs 82.1 (P less than .05); wheat, 79.6 vs 86.4 (P less than .05); corn gluten feed, 73.9 vs 70.9; rice bran, 57.3 vs 57.4; wheat middlings, 70.8 vs 79.4 (P less than .05); soybean meal, 89.9 vs 93.0; a mixture of soybean meal and hulls, 86.9 vs 88.8; coconut expeller, 65.0 vs 69.8; fababeans, 79.5 vs 78.5; peas, 87.6 vs 88.1; meat meal, 83.2 vs 84.0; complete diet (4.3% crude fiber), 76.3 vs 83.6 (P less than .05); and complete diet (13.6% crude fiber), 56.5 vs 55.0.(ABSTRACT TRUNCATED AT 250 WORDS)
用四头（50至70千克）公猪进行研究，以评估移动尼龙袋技术（MNBT）在测定多种饲料表观蛋白质消化率（AD）方面的效果。将每种饲料1克经1.0毫米筛网研磨后的样品装入尼龙袋（25×40毫米；48微米筛孔）。在进行预消化（0.01N盐酸；4000福林氏肠蛋白酶单位/升）后，通过十二指肠T形插管将每个样品的四个袋子插入每头猪体内。在进行粗蛋白分析之前，将未消化的内容物按猪和饲料进行汇集。每种饲料的AD先前已根据传统方法（CM）用四头公猪测定，即在14天适应期后进行5天粪便总收集。MNBT（n = 4）和CM（n = 4）分别得到以下结果，AD（%）：大麦，70.1对81.2（P＜0.05）；玉米，72.3对82.1（P＜0.05）；小麦，79.6对86.4（P＜0.05）；玉米麸质饲料，73.9对70.9；米糠，57.3对57.4；小麦次粉，70.8对79.4（P＜0.05）；豆粕，89.9对93.0；豆粕与豆皮混合物，86.9对88.8；椰子粕，65.0对69.8；蚕豆，79.5对78.5；豌豆，87.6对88.1；肉粉，83.2对84.0；全价日粮（4.3%粗纤维），76.3对83.6（P＜0.05）；全价日粮（13.6%粗纤维），56.5对55.0。（摘要截于250字）
