Huang Kai-Peng, Zhang Zhi-Hao, Li Rui-Ming, Chen Xiao
Department of Pharmacy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Evid Based Complement Alternat Med. 2016;2016:8562528. doi: 10.1155/2016/8562528. Epub 2016 Feb 29.
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that leads to severe multiorgan damage. Lang Chuang Fang (LCF) is a Chinese herbal medicine that is clinically prescribed for treating SLE. In this study, we examined the therapeutic effects of LCF granule on lupus-prone MRL/lpr mice. Female mice were randomly separated into six groups, and LCF treatment groups received LCF granule at the dosage of 0.97 g/kg/d, 1.95 g/kg/d, and 3.90 g/kg/d, respectively. Here, we found that, compared to the MRL/lpr mice, both the spleen coefficient and thymus coefficient were reduced in the LCF granule-treated mice. There was a marked downregulation in CRP and anti-dsDNA autoantibody and an evident upregulation of CH50 in LCF granule-treated mice. LCF granule treatment also obviously reduced the proteinuria, BUN, and SCr levels in MRL/lpr mice at the dosage of 0.97 g/kg/d, 1.95 g/kg/d, and 3.90 g/kg/d, indicating that LCF granule alleviated the renal injury of MRL/lpr mice. Furthermore, LCF granule decreased p65 NF-κB levels and increased Sirt1 and Nrf2 levels in the kidney tissues of MRL/lpr mice, which might elucidate the beneficial effects of LCF on lupus nephritis. In conclusion, this study demonstrates that LCF granule has therapeutic effects on lupus-prone MRL/lpr mice.
系统性红斑狼疮（SLE）是一种导致严重多器官损害的慢性自身免疫性疾病。狼疮方（LCF）是一种临床上用于治疗SLE的中药。在本研究中，我们检测了LCF颗粒对狼疮易感MRL/lpr小鼠的治疗效果。将雌性小鼠随机分为六组，LCF治疗组分别接受剂量为0.97 g/kg/d、1.95 g/kg/d和3.90 g/kg/d的LCF颗粒。在此，我们发现，与MRL/lpr小鼠相比，LCF颗粒治疗的小鼠脾脏系数和胸腺系数均降低。LCF颗粒治疗的小鼠中CRP和抗双链DNA自身抗体明显下调，CH50明显上调。LCF颗粒治疗还明显降低了剂量为0.97 g/kg/d、1.95 g/kg/d和3.90 g/kg/d的MRL/lpr小鼠的蛋白尿、血尿素氮和血肌酐水平，表明LCF颗粒减轻了MRL/lpr小鼠的肾损伤。此外，LCF颗粒降低了MRL/lpr小鼠肾组织中p65 NF-κB水平，提高了Sirt1和Nrf2水平，这可能解释了LCF对狼疮性肾炎的有益作用。总之，本研究表明LCF颗粒对狼疮易感MRL/lpr小鼠具有治疗作用。
