Shen N, Dagasan L, Sledjeski D, Weiner R M
Department of Microbiology, University of Maryland, College Park 20742.
J Bacteriol. 1989 Apr;171(4):2226-8. doi: 10.1128/jb.171.4.2226-2228.1989.
Separation on the basis of molecular weight resolved three proteins specific to the swarmer cell of Hyphomonas jannaschiana. In the reproductive cell, 4 major proteins were identified as cytoplasmic and 10 were identified as envelope. Of these envelope proteins, one was common to both the inner and outer membranes, four were common to the inner membrane, and five were common to the outer membrane. Four of these outer membrane proteins were specific to the reproductive cell, and two of these proteins, with apparent molecular weights of 116,000 and 29,000, constituted 19% of the total cell protein and 54% of the outer membrane protein.
基于分子量的分离解析出了詹氏海单胞菌游动细胞特有的三种蛋白质。在生殖细胞中,鉴定出4种主要蛋白质为细胞质蛋白,10种为包膜蛋白。在这些包膜蛋白中,一种在内膜和外膜中都有,4种在内膜中常见,5种在外膜中常见。这些外膜蛋白中有4种是生殖细胞特有的,其中两种蛋白,表观分子量分别为116,000和29,000,占细胞总蛋白的19%和外膜蛋白的54%。