Smith A M J, Bonato M, Dzama K, Malecki I A, Cloete S W P
Department of Animal Sciences, University of Stellenbosch, Matieland 7602, South Africa.
Department of Animal Sciences, University of Stellenbosch, Matieland 7602, South Africa.
Anim Reprod Sci. 2016 May;168:138-150. doi: 10.1016/j.anireprosci.2016.03.007. Epub 2016 Mar 16.
The success of assisted reproduction techniques is dependent on a sound foundation of understanding sperm characteristics to evaluate so as to improve semen processing. This study offers a descriptive basis for ostrich semen quality in terms of sperm function characteristics (SFC) that include motility, measured by computer assisted sperm analysis CASA (SCA(®)), viability (SYBR14/PI) and membrane integrity (hypo-osmotic swelling test). Relationships among these SFC's were explored and described by correlations and regressions. Certain fixed effects including the dilution of semen, season, year and male associated with semen collection were interpreted for future applications. The seasonal effect on sperm samples collected throughout the year suggested that it is prudent to restrict collections to spring and summer when SFC's and sperm concentration are maximized, compared to winter when these aspects of sperm quality are suppressed. Dilution of ejaculates helped to maintain important SFC's associated with fertilization success. The SFC's and sperm concentration varied among males, with specific males, having greater values for the percentage of motile (MOT) and progressively motile (PMOT) sperm, as well as sperm velocity (VCL, VSL, VAP) and linearity (LIN) variables. Males may thus be screened on these variables for inclusion in an artificial insemination (AI) programme to optimize fertility success rates.
辅助生殖技术的成功取决于对精子特征有充分的了解，以便评估并改进精液处理。本研究从精子功能特征（SFC）方面为鸵鸟精液质量提供了描述性依据，这些特征包括通过计算机辅助精子分析（CASA，SCA(®)）测量的活力、生存力（SYBR14/PI）和膜完整性（低渗肿胀试验）。通过相关性和回归分析探索并描述了这些SFC之间的关系。对包括精液稀释、季节、年份以及与精液采集相关的雄性等某些固定效应进行了解释，以供未来应用。全年采集的精子样本的季节效应表明，与精子质量这些方面受到抑制的冬季相比，将采集限制在春季和夏季是明智的，此时SFC和精子浓度最高。精液稀释有助于维持与受精成功相关的重要SFC。SFC和精子浓度在雄性之间存在差异，特定雄性的活动精子（MOT）和渐进性活动精子（PMOT）百分比以及精子速度（VCL、VSL、VAP）和线性度（LIN）变量的值更高。因此，可以根据这些变量对雄性进行筛选，以纳入人工授精（AI）计划，从而优化生育成功率。
