Ma Tao, Xiao Qiang, Yu Yu-Geng, Wang Cai, Zhu Cheng-Qi, Sun Zhao-Hui, Chen Xiao-Yang, Wen Xiu-Jun
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University , Guangzhou 510642, China.
Key Laboratory of Tea Plants Biology and Resources Utilization of Agriculture Ministry, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008, China.
J Agric Food Chem. 2016 Apr 27;64(16):3161-6. doi: 10.1021/acs.jafc.6b00339. Epub 2016 Apr 11.
The tea geometrid, Ectropis grisescens Warren, is one of the most severe defoliator insect pests in tea plantations, China. The use of insecticides, etc., is forbidden on organic tea plantations. No female-produced sex pheromones of E. grisescens had been previously identified. In the present study, female gland extracts were analyzed by gas chromatography coupled with electroantennographic detection (GC-EAD) and two-dimensional gas chromatography (GC×GC) using a time-of-flight mass spectrometric detector (TOFMS). Two components, (Z,Z,Z)-3,6,9-octadecatriene (Z3Z6Z9-18:Hy) and (Z,Z)-3,9-6,7-epoxyoctadecadiene (Z3Z9-6,7-epo-18:Hy), were identified from pheromone gland extracts, and their electrophysiological and behavioral activity evaluated. Under laboratory conditions, Z3Z9-6,7-epo-18:Hy elicited a stronger electrophysiological response than Z3Z6Z9-18:Hy. In the field, traps baited with Z3Z9-6,7-epo-18:Hy alone showed better results than traps baited with Z3Z6Z9-18:Hy, and the binary mixture of Z3Z9-6,7-epo-18:Hy and Z3Z6Z9-18:Hy in a ratio of 4:1 (approximate ratio of females emitting pheromone) caught more males than the single components or any other blends. This study showed that Z3Z6Z9-18:Hy and Z3Z9-6,7-epo-18:Hy are the sex pheromone components of E. grisescens and that they prove useful in developing alternative management tools for the pest.
茶尺蠖（Ectropis grisescens Warren）是中国茶园中最严重的食叶害虫之一。有机茶园禁止使用杀虫剂等。此前尚未鉴定出茶尺蠖雌虫产生的性信息素。在本研究中，利用气相色谱-触角电位检测联用仪（GC-EAD）和配备飞行时间质谱检测器（TOFMS）的二维气相色谱仪（GC×GC）对雌虫腺体提取物进行了分析。从性信息素腺体提取物中鉴定出两种成分，即（Z,Z,Z）-3,6,9-十八碳三烯（Z3Z6Z9-18:Hy）和（Z,Z）-3,9-6,7-环氧十八碳二烯（Z3Z9-6,7-epo-18:Hy），并对它们的电生理活性和行为活性进行了评估。在实验室条件下，Z3Z9-6,7-epo-18:Hy引发的电生理反应比Z3Z6Z9-18:Hy更强。在田间，仅用Z3Z9-6,7-epo-18:Hy诱捕的诱捕器比用Z3Z6Z9-18:Hy诱捕的诱捕器效果更好，且Z3Z9-6,7-epo-18:Hy与Z3Z6Z9-18:Hy按4:1的比例（近似雌虫释放性信息素的比例）混合的二元混合物捕获的雄虫比单一成分或其他任何混合物都多。本研究表明，Z3Z6Z9-18:Hy和Z3Z9-6,7-epo-18:Hy是茶尺蠖的性信息素成分，它们在开发该害虫的替代防治工具方面很有用。
