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昆虫中的化学感应基因家族及II型性信息素检测候选基因

Chemosensory Gene Families in and Candidates for Detection of Type-II Sex Pheromones.

作者信息

Li Zhao-Qun, Luo Zong-Xiu, Cai Xiao-Ming, Bian Lei, Xin Zhao-Jun, Liu Yan, Chu Bo, Chen Zong-Mao

机构信息

Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, China.

出版信息

Front Physiol. 2017 Nov 21;8:953. doi: 10.3389/fphys.2017.00953. eCollection 2017.

DOI:10.3389/fphys.2017.00953
PMID:29209233
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5702326/
Abstract

Tea grey geometrid (), a devastating chewing pest in tea plantations throughout China, produces Type-II pheromone components. Little is known about the genes encoding proteins involved in the perception of Type-II sex pheromone components. To investigate the olfaction genes involved in . sex pheromones and plant volatiles perception, we sequenced female and male antennae transcriptomes of . . After assembly and annotation, we identified 153 candidate chemoreception genes in , including 40 odorant-binding proteins (OBPs), 30 chemosensory proteins (CSPs), 59 odorant receptors (ORs), and 24 ionotropic receptors (IRs). The results of phylogenetic, qPCR, and mRNA abundance analyses suggested that three candidate pheromone-binding proteins (EgriOBP2, 3, and 25), two candidate general odorant-binding proteins (EgriOBP1 and 29), six pheromone receptors (EgriOR24, 25, 28, 31, 37, and 44), and EgriCSP8 may be involved in the detection of Type-II sex pheromone components. Functional investigation by heterologous expression in oocytes revealed that EgriOR31 was robustly tuned to the . sex pheromone component (Z,Z,Z)-3,6,9-octadecatriene and weakly to the other sex pheromone component (Z,Z)-3,9-6,7-epoxyoctadecadiene. Our results represent a systematic functional analysis of the molecular mechanism of olfaction perception in . with an emphasis on gene encoding proteins involved in perception of Type-II sex pheromones, and provide information that will be relevant to other Lepidoptera species.

摘要

茶尺蠖是中国各茶园中一种极具破坏力的咀嚼式害虫，能产生II型性信息素成分。目前对于编码参与II型性信息素成分感知的蛋白质的基因了解甚少。为了研究参与性信息素和植物挥发物感知的嗅觉基因，我们对茶尺蠖雌、雄触角转录组进行了测序。组装和注释后，我们在茶尺蠖中鉴定出153个候选化学感受基因，包括40个气味结合蛋白（OBP）、30个化学感受蛋白（CSP）、59个气味受体（OR）和24个离子型受体（IR）。系统发育分析、定量PCR和mRNA丰度分析结果表明，三个候选性信息素结合蛋白（EgriOBP2、3和25）、两个候选普通气味结合蛋白（EgriOBP1和29）、六个性信息素受体（EgriOR24、25、28、31、37和44）以及EgriCSP8可能参与II型性信息素成分的检测。通过在卵母细胞中进行异源表达的功能研究表明，EgriOR31对性信息素成分（Z,Z,Z）-3,6,9-十八碳三烯反应强烈，而对另一种性信息素成分（Z,Z）-3,9-6,7-环氧十八碳二烯反应较弱。我们的研究结果代表了对茶尺蠖嗅觉感知分子机制的系统功能分析，重点关注编码参与II型性信息素感知的蛋白质的基因，并提供了与其他鳞翅目物种相关的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/3224da588b78/fphys-08-00953-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/a3509d646d96/fphys-08-00953-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/d82a3d64f337/fphys-08-00953-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/c34a5c7543fa/fphys-08-00953-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/7676ef171dff/fphys-08-00953-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/ca6c4390d3c7/fphys-08-00953-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/40d743ae8f72/fphys-08-00953-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/3477cefce582/fphys-08-00953-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/033c7e50c1ff/fphys-08-00953-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/3224da588b78/fphys-08-00953-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/a3509d646d96/fphys-08-00953-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/d82a3d64f337/fphys-08-00953-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/c34a5c7543fa/fphys-08-00953-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/7676ef171dff/fphys-08-00953-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/ca6c4390d3c7/fphys-08-00953-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/40d743ae8f72/fphys-08-00953-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/3477cefce582/fphys-08-00953-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/033c7e50c1ff/fphys-08-00953-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3ac/5702326/3224da588b78/fphys-08-00953-g0009.jpg

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