聚合酶链反应在人类胃部疾病幽门螺杆菌DNA检测中的临床意义
Clinical significance of PCR in Helicobacter pylori DNA detection in human gastric disorders.
作者信息
Xu G M, Ji X H, Li Z S, Man X H, Zhang H F
机构信息
Guo-Ming Xu, Xu-Huai Ji, Zhao-Shen Li, Xiao-Hua Man, Hong-Fu Zhang, Department of Gastroenterology, Changhai Hospital, Second Military Medic al University, Shanghai 200433, China.
出版信息
World J Gastroenterol. 1997 Jun 15;3(2):98-100. doi: 10.3748/wjg.v3.i2.98.
AIM
To investigate the clinical significance of the PCR assay in the diagnosis of gastric Helicobacter pylori (Hp) infection.
METHODS
Hp infection in gastric antral biopsied specimens was identified by using the polymerase chain reaction (PCR) to amplify the specific Hp urease gene fragments (PCR-Hp-DNA) in 154 patients with gastrointestinal disorders. Hp urease gene oligonucleotide primers specific for Hp (16s rRNA) were used. Urease test and enzyme-linked immunosorbent assay (ELISA) for anti Hp-IgG serum were also used as controls.
RESULTS
PCR-Hp-DNA was detected in 140 (91%) of the 154 patients, where patients 114 and 125 were found infected with Hp by urease test and ELISA Hp IgG, respectively. There was a marked difference in the Hp-positive rate between the PCR-Hp-DNA and the urease test or ELISA-Hp-IgG (P < 0.05). The Hp infection rate increased with age, although a minority of infected people developed signs and symptoms of gastric disorders. Hp infection is closely related to adenocarcinoma in both the gastric antrum as well as the down body of the stomach.
CONCLUSION
PCR is a sensitive and specific method for the detection of Hp in human gastric tissues. Detection of Hp DNA in vivo using this approach might improve the clinical diagnosis and epidemiological research related to H. pylori infection.
目的
探讨聚合酶链反应（PCR）检测法在诊断胃幽门螺杆菌（Hp）感染中的临床意义。
方法
采用聚合酶链反应（PCR）扩增154例胃肠道疾病患者胃窦活检标本中的特异性Hp脲酶基因片段（PCR-Hp-DNA），以鉴定Hp感染情况。使用针对Hp（16s rRNA）的脲酶基因寡核苷酸引物。同时采用脲酶试验和抗Hp-IgG血清酶联免疫吸附测定（ELISA）作为对照。
结果
154例患者中，140例（91%）检测到PCR-Hp-DNA，其中患者114和125分别通过脲酶试验和ELISA Hp IgG检测出感染Hp。PCR-Hp-DNA与脲酶试验或ELISA-Hp-IgG之间的Hp阳性率存在显著差异（P<0.05）。Hp感染率随年龄增加而升高，尽管少数感染者出现胃部疾病的体征和症状。Hp感染与胃窦及胃体下部的腺癌密切相关。
结论
PCR是检测人胃组织中Hp的一种灵敏且特异的方法。采用该方法体内检测Hp DNA可能会改善与幽门螺杆菌感染相关的临床诊断和流行病学研究。
Zotero 插件