Chen Jie, Yu Yan-ping, Huang Xiao-lei, Chen Fei-bo
Department of Gastroenterology, Affiliated Children's Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
Zhonghua Er Ke Za Zhi. 2003 Mar;41(3):168-71.
To determine the changes of IL-8 and IL-8 mRNA in gastric and duodenal mucosa of children with Hp infection, to study the effect of Helicobacter pylori (Hp) infection on the expression of IL-8 and IL-8 mRNA, and to evaluate its possible roles in the pathogenesis of gastric and duodenal mucosal inflammation in Hp related gastroduodenal diseases.
Biopsy specimens were taken from the antral and duodenal mucosa on endoscopy in patients with or without Hp infection, which was diagnosed by urease test and Warshing-Starry staining. The expression of IL-8 in gastric and duodenal mucosa was determined by ELISA, the expression of IL-8 mRNA was determined by using RT-PCR.
Inflammation of gastric antral mucosa was more severe in Hp-positive group than in Hp-negative group. Active inflammation often existed on the basis of chronic inflammation in Hp-positive mucosa, and duodenal mucosa had mild chronic inflammation in Hp-positive group. Of 17 children who were not infected with Hp, 4 had pathologically normal gastric mucosa and had mild chronic gastritis, one child had an active chronic gastritis. Nineteen children were infected with Hp and all had chronic gastritis with signs of active inflammation. Gastric and duodenal mucosal IL-8 and IL-8 mRNA were higher in HP infected than in non infected children (IL-8: in gastric mucosa 24.66 - 177.77 pg/mg, 2.94 - 12.98 pg/mg, t = 12.34, P < 0.01; in duodenal mucosa: 4.28 - 47.76 pg/mg, 2.04 - 9.52 pg/mg, t = 7.18, P < 0.01. IL-8 mRNA: in gastric mucosa 2.37 - 4.99, 0.05 - 0.44, t = 29.29, P < 0.01; in duodenal mucosa 1.22 - 1.87, 0.01 - 0.23, t = 37.20, P < 0.01). Children with active chronic gastritis had higher interleukin-8 levels and IL-8 mRNA expression than those with inactive gastritis (IL-8 in gastric mucosa: 12.98 - 177.77 pg/mg, 2.04 - 10.43 pg/mg, t = 10.66, P < 0.01; in duodenal mucosa: 5.28 - 47.76 pg/mg, 3.19 - 8.14 pg/mg, t = 6.52, P < 0.01. IL-8 mRNA in gastric mucosa: 0.51 - 4.99, 0.01 - 0.44, t = 18.62, P < 0.01; in duodenal mucosa: 0.23 - 1.87, 0.01 - 0.20, t = 19.10, P < 0.01).
Higher expression of IL-8 and IL-8 mRNA was seen in Hp-positive gastric and duodenal mucosa and in active gastritis. IL-8 may play an important role in the local gastric and duodenal mucosal inflammatory infiltration with a large number of neutrophils when there is Hp infection.
检测幽门螺杆菌（Hp）感染患儿胃及十二指肠黏膜中白细胞介素-8（IL-8）及IL-8 mRNA的变化，研究Hp感染对IL-8及IL-8 mRNA表达的影响，评估其在Hp相关性胃十二指肠疾病胃及十二指肠黏膜炎症发病机制中的可能作用。
对经尿素酶试验及Warshing-Starry染色确诊为有无Hp感染的患者，在内镜下取胃窦及十二指肠黏膜活检标本。采用酶联免疫吸附测定法（ELISA）检测胃及十二指肠黏膜中IL-8的表达，采用逆转录聚合酶链反应（RT-PCR）检测IL-8 mRNA的表达。
Hp阳性组胃窦黏膜炎症较Hp阴性组更严重。Hp阳性黏膜的活动性炎症常存在于慢性炎症基础上，Hp阳性组十二指肠黏膜有轻度慢性炎症。17例未感染Hp的儿童中，4例胃黏膜病理正常，有轻度慢性胃炎，1例为活动性慢性胃炎。19例感染Hp的儿童均有慢性胃炎且有活动性炎症表现。Hp感染儿童胃及十二指肠黏膜IL-8及IL-8 mRNA水平高于未感染儿童（IL-8：胃黏膜中24.66 - 177.77 pg/mg，2.94 - 12.98 pg/mg，t = 12.34，P < 0.01；十二指肠黏膜中：4.28 - 47.76 pg/mg，2.04 - 9.52 pg/mg，t = 7.18，P < 0.01。IL-8 mRNA：胃黏膜中2.37 - 4.99，0.05 - 0.44，t = 29.29，P < 0.01；十二指肠黏膜中1.22 - 1.87，0.01 - 0.23，t = 37.20，P < 0.01）。活动性慢性胃炎患儿白细胞介素-8水平及IL-8 mRNA表达高于非活动性胃炎患儿（胃黏膜中IL-8：12.98 - 177.77 pg/mg，2.04 - 10.43 pg/mg，t = 10.66，P < 0.01；十二指肠黏膜中：5.28 - 47.76 pg/mg，3.19 - 8.14 pg/mg，t = 6.52，P < 0.01。胃黏膜中IL-8 mRNA：0.51 - 4.99，0.01 - 0.44，t = 18.62，P < 0.01；十二指肠黏膜中：0.23 - 1.87，0.01 - 0.20，t = 19.10，P < 0.01）。
Hp阳性胃及十二指肠黏膜及活动性胃炎中IL-8及IL-8 mRNA表达升高。当存在Hp感染时，IL-8可能在胃及十二指肠局部黏膜炎症中大量中性粒细胞浸润中起重要作用。
