慢性肝病患者肝细胞中胰岛素样生长因子II及其受体的表达
Expression of insulin-like growth factor II and its receptor in liver cells from patients with chronic liver diseases.
作者信息
Yang D H, Xiu C, Yang B, Gu J R, Qian L F, Qu S M
机构信息
Dong-Hua Yang, Chong Xiu, Bo Yang, Department of Gastroenterology, Zhujiang Hospital, The First Military Medical University, Guangzhou 510282, Guangdong Province, China.
出版信息
World J Gastroenterol. 1997 Jun 15;3(2):117-8. doi: 10.3748/wjg.v3.i2.117.
AIM
To study the relationship between insulin-like growth factor II (IGF-II), IGF-II receptor, and chronic liver diseases and to investigate the clinical mechanisms of human hepatocellular carcinoma (HCC) development.
METHODS
We analyzed IGF-II and IGF-II receptor poly (A)+ mRNA in dysplasia liver cell (DLC; n = 10), liver cirrhosis (LC; n = 9), and chronic active hepatitis (CAH; n = 9) specimens by Northern blot using human IGF-II and IGF-II receptor DNA probes labeled with (32) P through nick translation.
RESULTS
Expression of IGF-II in DLC samples (10/10, 100%) was higher than in CAH (3/9, 33%) and LC samples (3/9, 33%) (P < 0.01). Expression of IGF-II receptor in DLC samples (7/10, 70%) was significantly higher than in CAH (2/9, 22%) and LC samples (3/9, 33%). Data on hepatitis B virus (HBV) infection status from different chronic liver disease samples were also analyzed.
CONCLUSION
Overexpression of IGF-II and IGF-II receptor in DLC samples was associated with a preceding step to malignant phenotype hepatocyte transformation and may be of diagnostic value for early detection of hepatocellular carcinoma (HCC). Persistent HBV infection was strongly associated with abnormal IGF-II and IGF-II receptor mRNA expression, suggesting that an autocrine or paracrine mechanism is involved in the regulation of growth in liver cell carcinogenesis.
目的
研究胰岛素样生长因子II（IGF-II）、IGF-II受体与慢性肝病之间的关系，并探讨人类肝细胞癌（HCC）发生发展的临床机制。
方法
我们通过Northern印迹法，使用经缺口平移法用（32）P标记的人IGF-II和IGF-II受体DNA探针，分析发育异常肝细胞（DLC；n = 10）、肝硬化（LC；n = 9）和慢性活动性肝炎（CAH；n = 9）标本中IGF-II和IGF-II受体聚腺苷酸加尾（poly (A)+）mRNA。
结果
DLC样本中IGF-II的表达（10/10，100%）高于CAH样本（3/9，33%）和LC样本（3/9，33%）（P < 0.01）。DLC样本中IGF-II受体的表达（7/10，70%）显著高于CAH样本（2/9，22%）和LC样本（3/9，33%）。还分析了来自不同慢性肝病样本的乙型肝炎病毒（HBV）感染状态数据。
结论
DLC样本中IGF-II和IGF-II受体的过表达与肝细胞向恶性表型转化的前期步骤相关，可能对肝细胞癌（HCC）的早期检测具有诊断价值。持续性HBV感染与IGF-II和IGF-II受体mRNA异常表达密切相关，提示自分泌或旁分泌机制参与肝细胞癌发生过程中的生长调节。
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