Seo J H, Kim K W, Murakami S, Park B C
Department of Molecular Biology, Pusan National University, Korea.
J Korean Med Sci. 1997 Dec;12(6):523-31. doi: 10.3346/jkms.1997.12.6.523.
To evaluate the possibility that HBxAg is related to an enhanced expression of IGF-II, immunohistochemical staining was performed for distribution and colocalization of HBxAg and IGF-II in liver tissues from 40 chronic active hepatitis (CAH-B), 51 cirrhosis and 46 hepatocellular carcinoma (HCC) patients using polyclonal rabbit anti HBxAg raised against full length-recombinant HBxAg and monoclonal mouse anti IGF-II. HBxAg in CAH-B, cirrhosis and HCC tissues was detected in 95%, 39% and 17%, whereas IGF-II in the same tissues was seen in 0%, 92% and 100%, respectively. There was a gradual decrease in the prevalence of HBxAg expression in cirrhosis and HCC, as compared to CAH-B tissues. All of the cirrhosis and HCC samples with positive staining for HBxAg expressed IGF-II. However, 55% of cirrhosis and 100% of HCC samples without HBxAg staining also expressed IGF-II. Moreover, colocalization at neighboring sections, even in both HBxAg and IGF-II positive samples, was not regularly observed. It is concluded that HBxAg expression in CAH-B may play a role in the pathogenesis of CAH-B. Although HBxAg may be related to the expression of IGF-II in some cirrhotic and HCC tissues, IGF-II expression in a large majority of these cases may be related to other factor(s) than HBxAg.
为评估乙肝病毒X抗原（HBxAg）与胰岛素样生长因子-II（IGF-II）表达增强之间的相关性，我们使用针对全长重组HBxAg的兔多克隆抗HBxAg抗体和小鼠抗IGF-II单克隆抗体，对40例慢性活动性乙型肝炎（CAH-B）患者、51例肝硬化患者和46例肝细胞癌（HCC）患者的肝组织进行了免疫组化染色，以检测HBxAg和IGF-II的分布及共定位情况。CAH-B、肝硬化和HCC组织中HBxAg的检出率分别为95%、39%和17%，而相同组织中IGF-II的检出率分别为0%、92%和100%。与CAH-B组织相比，肝硬化和HCC组织中HBxAg表达的患病率逐渐降低。所有HBxAg染色阳性的肝硬化和HCC样本均表达IGF-II。然而，55%的肝硬化样本和100%的无HBxAg染色的HCC样本也表达IGF-II。此外，即使在HBxAg和IGF-II均为阳性的相邻切片中，也未经常观察到共定位现象。结论是，CAH-B中HBxAg的表达可能在CAH-B的发病机制中起作用。虽然HBxAg可能在某些肝硬化和HCC组织中与IGF-II的表达有关，但在大多数这些病例中，IGF-II的表达可能与HBxAg以外的其他因素有关。
