Chen Dahui, Sun Hao, Shen Jiaqi, Igor Longo Phemba, Zheng Xiaoyong, Hu Shuping, Xiang Zheng
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
School of International Studies, Wenzhou Medical University, Wenzhou 325035, China.
J Chromatogr Sci. 2016 Aug;54(7):1166-70. doi: 10.1093/chromsci/bmw044. Epub 2016 Apr 4.
Rosavin is a bioactive antidepressant component isolated from Rhodiola rosea L. In this work, an ultra-performance liquid chromatography (UPLC) method was established for the determination of rosavin in rat plasma. The chromatographic separation was achieved on a HSS T3 column (100 mm × 2.1 mm, 1.8 μm) with a gradient mobile phase consisting of acetonitrile and water (0.1% formic acid). Plasma samples were processed with one-step protein precipitation. Rutin was chosen as internal standard and the detection wavelength was 249 nm. The pharmacokinetic parameters were analyzed using the drug and statistics software. The results showed that the established method has an excellent linearity in the range of 10-1,000 ng/mL (R(2) = 0.992) with a lower limit of quantification (10 ng/mL). The intra- and interday precision (relative standard deviation) were from 2.0 to 10.6% and the extraction recovery was 92.4-95.1%. The simple and rapid UPLC method was successfully applied to the pharmacokinetic and bioavailability study of rosavin in rats.
红景天苷是从蔷薇红景天中分离出的一种具有生物活性的抗抑郁成分。在本研究中，建立了一种超高效液相色谱（UPLC）法用于测定大鼠血浆中的红景天苷。色谱分离在HSS T3柱（100 mm×2.1 mm，1.8μm）上进行，流动相为乙腈和水（含0.1%甲酸）的梯度洗脱。血浆样品采用一步蛋白沉淀法处理。选用芦丁作为内标，检测波长为249 nm。使用药物与统计学软件分析药代动力学参数。结果表明，所建立的方法在10 - 1000 ng/mL范围内具有良好的线性关系（R(2) = 0.992），定量下限为10 ng/mL。日内和日间精密度（相对标准偏差）为2.0%至10.6%，提取回收率为92.4%至95.1%。该简单快速的UPLC方法成功应用于红景天苷在大鼠体内的药代动力学和生物利用度研究。
