Ma D L, Li J Y, Liu Y E, Liu C M, Li J, Lin G Z, Yan J
Department of Oncology, Yishui Central Hospital of Linyi, Linyi, China.
Department of Infectious Disease, Yishui Central Hospital of Linyi, Linyi, China.
J Biol Regul Homeost Agents. 2016 Jan-Mar;30(1):91-102.
This study was carried out to acquire solid evidence that some common treatments could affect micro ribonucleic acids (miRNAs) by revealing the regulatory effect of genes, so as to provide a reference for further exploration of the prevention and treatment of cervical cancer. Nude mouse tumorigenicity assay was used to study the effect of inhibiting miR-574-5p on development and tumorigenic ability of Henrietta Lacks (HeLa) tumor. Cell wound scratch assay, flow cytometry and real-time quantitative polymerase chain reaction (RT-qPCR) were adopted to study the effects of anoxia and temperature, etc., on expression of miR-574-5p and QKI in HeLa as well as on the clone and migration ability of cells, to provide prevention and treatment of cervical cancer with new ideas and evidence. The results demonstrated that cervical cancer tissues had a significantly increased miR-574-5p expression compared with para-carcinoma tissues; conversely, Gomafu, overall QKI (pan-QKI) and QKI-5 messenger ribonucleic acid (mRNA) and protein expression all decreased. Part of the common nursing methods had a certain influence on miR-574-5p expression, HeLa reproduction and metastasis, and even cell cycle. For example, ultraviolet (UV) irradiation was effective in decreasing miR-574-5p expression of HeLa and inhibiting cell migration; severe hypoxia significantly decreased the survival rate of HeLa, leading to the increase of programmed death percentage and cell ratio in G2/M phase as well as the decrease of cell ratio in G1 phase. Incubation at different temperatures also affected miR-574-5p expression and cell proliferation. Thus, it can be known that miR-574-5p, Gomafu and QKI expression in cervical cancer tissues and para-carcinoma tissues are significantly up-regulated or down-regulated. Some treatments, such as UV irradiation, hypoxia, incubation temperatures, etc., can affect miR-574-5p expression and HeLa proliferation as well as metastases in different degrees. These findings provide a reference and basis for further study.
本研究旨在通过揭示基因的调控作用，获取一些常见治疗方法可影响微小核糖核酸（miRNA）的确凿证据，从而为进一步探索宫颈癌的防治提供参考。采用裸鼠致瘤性试验研究抑制miR-574-5p对海拉（HeLa）肿瘤生长和致瘤能力的影响。采用细胞划痕试验、流式细胞术和实时定量聚合酶链反应（RT-qPCR）研究缺氧、温度等对HeLa细胞中miR-574-5p和QKI表达以及细胞克隆和迁移能力的影响，为宫颈癌的防治提供新思路和证据。结果表明，与癌旁组织相比，宫颈癌组织中miR-574-5p表达显著升高；相反，Gomafu、总QKI（泛QKI）以及QKI-5信使核糖核酸（mRNA）和蛋白表达均降低。部分常见护理方法对miR-574-5p表达、HeLa细胞增殖和转移甚至细胞周期有一定影响。例如，紫外线（UV）照射可有效降低HeLa细胞中miR-574-5p表达并抑制细胞迁移；严重缺氧显著降低HeLa细胞存活率，导致程序性死亡百分比和G2/M期细胞比例增加以及G1期细胞比例降低。在不同温度下培养也会影响miR-574-5p表达和细胞增殖。因此，可以得知宫颈癌组织和癌旁组织中miR-574-5p、Gomafu和QKI表达显著上调或下调。一些治疗方法，如UV照射、缺氧、培养温度等，可不同程度地影响miR-574-5p表达以及HeLa细胞增殖和转移。这些发现为进一步研究提供了参考和依据。
