Hill R, Canal A, Bondioli K, Morell R, Garcia M D
School of Animal Sciences, Louisiana State University, LSU AgCenter, Baton Rouge, LA, USA.
Genet Mol Res. 2016 Mar 31;15(1):gmr7794. doi: 10.4238/gmr.15017794.
The objective of the present study was to investigate single nucleotide polymorphisms (SNPs) located in three candidate genes previously reported to have effects on fertility and milk production traits in a population of 123 Holstein cows. The milk production traits evaluated included lifetime averages of milk yield, protein concentration, and fat concentration. Fertility traits evaluated included lifetime averages of services per conception and days-open. Candidate genes included those encoding diacylglycerol acyltransferase (DGAT1), leptin receptor (LEPR), and calpastatin (CAST). A total of 60 SNPs were selected (20 per gene) at equidistant locations on each candidate gene to identify potential linkage with causative mutations. Four SNPs were identified as being significantly associated with the evaluated fertility traits. Specifically, SNPs rs109663724 and rs137673193 were significantly associated with lifetime average days-open, while rs109663724 and rs135560721 were significantly associated with lifetime average number of services per conception. Five SNP (rs109663724, rs132699547, rs135423283, rs135576599, and rs13675432) were significantly associated with lifetime averages of milk protein concentration and milk fat concentration, with only one SNP (rs109663724) being significantly associated with the average lifetime milk yield. Although multiple SNPs were identified in the current study as being significantly associated with milk production and fertility traits, it is essential that these SNPs are validated in larger populations, under more diverse environments, and that additional SNPs and candidate genes are evaluated prior to their implementation into selection strategies.
本研究的目的是在123头荷斯坦奶牛群体中，调查先前报道的对繁殖力和产奶性状有影响的三个候选基因中的单核苷酸多态性（SNP）。评估的产奶性状包括终身平均产奶量、蛋白质浓度和脂肪浓度。评估的繁殖力性状包括每次受孕的终身平均配种次数和空怀天数。候选基因包括编码二酰基甘油酰基转移酶（DGAT1）、瘦素受体（LEPR）和钙蛋白酶抑制蛋白（CAST）的基因。在每个候选基因上等距位置共选择了60个SNP（每个基因20个），以确定与致病突变的潜在连锁关系。鉴定出4个SNP与评估的繁殖力性状显著相关。具体而言，SNP rs109663724和rs137673193与终身平均空怀天数显著相关，而rs109663724和rs135560721与终身平均每次受孕配种次数显著相关。5个SNP（rs109663724、rs132699547、rs135423283、rs135576599和rs13675432）与终身平均乳蛋白浓度和乳脂肪浓度显著相关，只有1个SNP（rs109663724）与终身平均产奶量显著相关。尽管在本研究中鉴定出多个SNP与产奶和繁殖力性状显著相关，但至关重要的是，这些SNP要在更大的群体中、在更多样化的环境中进行验证，并且在将其纳入选择策略之前，要评估更多的SNP和候选基因。
