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本文引用的文献

1
Mechanisms of Bone Resorption in Periodontitis.牙周炎中骨吸收的机制
J Immunol Res. 2015;2015:615486. doi: 10.1155/2015/615486. Epub 2015 May 3.
2
Porphyromonas gingivalis Suppresses Differentiation and Increases Apoptosis of Osteoblasts From New Zealand Obese Mice.牙龈卟啉单胞菌抑制新西兰肥胖小鼠成骨细胞的分化并增加其凋亡。
J Periodontol. 2015 Sep;86(9):1095-102. doi: 10.1902/jop.2015.150032. Epub 2015 May 8.
3
Periodontitis: from microbial immune subversion to systemic inflammation.牙周炎：从微生物免疫颠覆到全身炎症
Nat Rev Immunol. 2015 Jan;15(1):30-44. doi: 10.1038/nri3785.
4
Porphyromonas gingivalis infection increases osteoclastic bone resorption and osteoblastic bone formation in a periodontitis mouse model.牙龈卟啉单胞菌感染增加牙周炎小鼠模型中破骨细胞的骨吸收和成骨细胞的骨形成。
BMC Oral Health. 2014 Jul 15;14:89. doi: 10.1186/1472-6831-14-89.
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Alveolar bone loss: mechanisms, potential therapeutic targets, and interventions.牙槽骨丧失：机制、潜在治疗靶点及干预措施
Adv Dent Res. 2014 May;26(1):38-46. doi: 10.1177/0022034514529305.
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Alcoholic and isocaloric diet, but not ovariectomy, influence the apoptosis of bone cells within the alveolar bone crest of rats.
Arch Oral Biol. 2014 Apr;59(4):424-33. doi: 10.1016/j.archoralbio.2014.01.008. Epub 2014 Jan 31.
7
Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells.牙龈卟啉单胞菌脂多糖抑制人牙周膜干细胞的成骨细胞分化并促进促炎细胞因子的产生。
Arch Oral Biol. 2014 Feb;59(2):167-75. doi: 10.1016/j.archoralbio.2013.11.008. Epub 2013 Nov 23.
8
A specific oligodeoxynucleotide promotes the differentiation of osteoblasts via ERK and p38 MAPK pathways.一种特定的寡脱氧核苷酸通过ERK和p38丝裂原活化蛋白激酶途径促进成骨细胞的分化。
Int J Mol Sci. 2012;13(7):7902-7914. doi: 10.3390/ijms13077902. Epub 2012 Jun 25.
9
An oligodeoxynucleotide that induces differentiation of bone marrow mesenchymal stem cells to osteoblasts in vitro and reduces alveolar bone loss in rats with periodontitis.一种在体外诱导骨髓间充质干细胞分化为成骨细胞并减少牙周炎大鼠牙槽骨丢失的寡脱氧核苷酸。
Int J Mol Sci. 2012;13(3):2877-2892. doi: 10.3390/ijms13032877. Epub 2012 Mar 5.
10
An oligodeoxynucleotide with promising modulation activity for the proliferation and activation of osteoblast.一种对成骨细胞的增殖和激活具有良好调节活性的寡脱氧核苷酸。
Int J Mol Sci. 2011;12(4):2543-55. doi: 10.3390/ijms12042543. Epub 2011 Apr 15.

[特异性序列寡脱氧核苷酸MT01对牙龈卟啉单胞菌侵袭的成骨细胞增殖、凋亡及细胞周期的影响]

[Effect of specific sequence oligodeoxynucleotide MT01 on the proliferation, apoptosis, and cell cycle of osteoblasts invaded by Porphyromonas gingivalis].

作者信息

Haijiao Yu, Yuqin Shen, Yin Liu, Han Gao, Yue Zhou, Tianqi Hu, Chongtao Lin

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2015 Dec;33(6):617-21. doi: 10.7518/hxkq.2015.06.014.

DOI:10.7518/hxkq.2015.06.014
PMID:27051956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7030364/
Abstract

OBJECTIVE

This aimed to investigate the effect of specific sequence oligodeoxynucleotide MT01 on the biological properties of osteoblasts invaded by Porphyromonas gingivalis (P. gingivalis ) by evaluating proliferation, cell cycle, and apoptosis.

METHODS

MG63 osteoblasts were recovered and incubated with MT01, CpG ODN, metronidazole (MNZ), and gentamicin (GEN) for 3 h. P. gingivalis (the multiplicity of infection was 100:1) was added subsequently and cocultured for another 24 and 48 h. Cells with PBS comprised the blank group, whereas cells with P. gingivalis comprised the negative controls. Six experimental groups were established: PBS group, P. gingivalis group, MT01+P. gingivalis group, CpG ODN+ P. gingivalis group, MNZ+P. gingivalis group, and GEN+P. gingivalis group. The proliferative ability was measured by methyl thiazolyl tetrazolium assay, and the percentages of apoptosis and cell cycle were examined by flow cytometry.

RESULTS

Compared with the blank group, proliferation increased significantly in the MT01+P. gingivalis group (P < 0.05). The ratio of cells was lower at the G₁ phase and higher at the S phase in the MT01+P. gingivalis group compared with the results in the P. gingivalis group (P < 0.05). Early cell apoptosis in the MT01+P. gingivalis group was significantly lower than that in the P. gingivalis group (P < 0.05).

CONCLUSION

MT01 can promote the proliferation, reduce the ratio of the G₁phase, increase the ratio of the S phase, and inhibit the early apoptosis of osteoblasts invaded by P. gingivalis.

摘要

目的

通过评估增殖、细胞周期和凋亡情况，研究特定序列寡脱氧核苷酸MT01对牙龈卟啉单胞菌（P. gingivalis）侵袭的成骨细胞生物学特性的影响。

方法

复苏MG63成骨细胞，分别与MT01、CpG ODN、甲硝唑（MNZ）和庆大霉素（GEN）孵育3小时。随后加入牙龈卟啉单胞菌（感染复数为100:1），再共培养24和48小时。用PBS处理的细胞作为空白组，用牙龈卟啉单胞菌处理的细胞作为阴性对照组。建立六个实验组：PBS组、牙龈卟啉单胞菌组、MT01 + 牙龈卟啉单胞菌组、CpG ODN + 牙龈卟啉单胞菌组、MNZ + 牙龈卟啉单胞菌组和GEN + 牙龈卟啉单胞菌组。采用甲基噻唑基四氮唑法测定增殖能力，通过流式细胞术检测凋亡率和细胞周期。

结果

与空白组相比，MT01 + 牙龈卟啉单胞菌组的增殖显著增加（P < 0.05）。与牙龈卟啉单胞菌组相比，MT01 + 牙龈卟啉单胞菌组G₁期细胞比例较低，S期细胞比例较高（P < 0.05）。MT01 + 牙龈卟啉单胞菌组的早期细胞凋亡明显低于牙龈卟啉单胞菌组（P < 0.05）。

结论

MT01可促进牙龈卟啉单胞菌侵袭的成骨细胞增殖，降低G₁期比例，增加S期比例，并抑制早期凋亡。