Luminex xTAG-GPP（胃肠道病原体检测试剂盒）在急性腹泻疾病诊断中的评估

Evaluation of the Luminex xTAG-GPP (Gastrointestinal Pathogen Panel) in the diagnosis of diseases with acute diarrhoea.

作者信息

Casañ Cristina, Ocete María Dolores, Medina Rafael, Gimeno Concepción

机构信息

Área Clínica de Microbiología, Enfermedades Infecciosas y Medicina Preventiva, Servicio de Microbiología, Consorcio Hospital General Universitario de Valencia, Valencia, España.

出版信息

Enferm Infecc Microbiol Clin. 2017 Nov;35(9):574-577. doi: 10.1016/j.eimc.2016.02.022. Epub 2016 Apr 6.

DOI:10.1016/j.eimc.2016.02.022

PMID:27059749

Abstract

INTRODUCTION

Most Microbiology laboratories use different techniques for the diagnosis of gastrointestinal infections. Some of which require at least 72 hours to obtain final results.

MATERIAL AND METHODS

The gastrointestinal panel Luminex (xTAG-GPP, Luminex Molecular Diagnostics, Toronto, Canada) is a qualitative multiplex fast and sensitive assay able to detect and to identify the 15 most common pathogens causing gastrointestinal infection simultaneously. We evaluated this multiplex panel comparing it with conventional methods used in our laboratory.

RESULTS

We analyzed 225 samples of feces. Through the conventional methods were positive 74 samples (32.9%). Through the Luminex method were positive 137 samples (60.9%).

CONCLUSIONS

The use of the xTAG GPP system in Clinical Microbiology can improve the diagnosis of gastrointestinal infectious because it provides results in less than 8 hours. Some pathogens should be applied with caution and should be interpreted based on the patient́s clinical data.

摘要