Deng Jiankai, Luo Xin, Wang Ruilian, Jiang Lingxiao, Ding Xixia, Hao Wei, Peng Yongzheng, Jiang Changhong, Yu Nan, Che Xiaoyan
Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, No. 253 Gong-ye Avenue, Guangzhou, 510282, PR China.
Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, No. 253 Gong-ye Avenue, Guangzhou, 510282, PR China.
Diagn Microbiol Infect Dis. 2015 Nov;83(3):325-30. doi: 10.1016/j.diagmicrobio.2015.07.024. Epub 2015 Jul 30.
To investigate whether Luminex xTAG® Gastrointestinal Pathogen Panel (xTAG GPP) is applicable for the diagnosis of diarrhea and surveillance of enteropathogens circulating in Southern China, 290 stool samples were tested for 15 kinds of enteropathogens using xTAG GPP and compared to the results from the routine tests, including culture; immunochromatography; real-time PCR; microscopy; and a third method, gene sequencing. One hundred fifty-nine samples were positive, yielding a total of 181 enteropathogens (69 bacteria and 112 viruses), with rotavirus being most prevalent (39.0%, 62/159). The overall sensitivity and specificity of xTAG GPP were 96.3% (93.3-98.2%) and 99.8% (99.6-99.9%), respectively, with a combination of the methods as the gold standard. The coinfection rates detected by the routine tests and xTAG GPP were 10.0% (25 double and 4 triple infections) and 12.1% (29 double, 4 triple and 2 quadruple infections), respectively. xTAG GPP is a powerful tool for the identification of multiple enteropathogens.
为研究Luminex xTAG®胃肠道病原体检测试剂盒（xTAG GPP）是否适用于中国南方腹泻的诊断及肠道病原体的监测，采用xTAG GPP对290份粪便样本进行了15种肠道病原体检测，并与常规检测结果进行比较，常规检测方法包括培养、免疫层析、实时荧光定量PCR、显微镜检查以及第三种方法基因测序。159份样本呈阳性，共检出181种肠道病原体（69种细菌和112种病毒），其中轮状病毒最为常见（39.0%，62/159）。以多种方法联合检测结果为金标准时，xTAG GPP的总体灵敏度和特异度分别为96.3%（93.3 - 98.2%）和99.8%（99.6 - 99.9%）。常规检测和xTAG GPP检测到的混合感染率分别为10.0%（25例双重感染和4例三重感染）和12.1%（29例双重感染、4例三重感染和2例四重感染）。xTAG GPP是鉴定多种肠道病原体的有力工具。
