Lehmann Caroline, Sieg Holger, Lampen Alfonso, Braeuning Albert
Department of Food Safety, Federal Institute for Risk Assessment, 10589 Berlin, Germany.
Department of Food Safety, Federal Institute for Risk Assessment, 10589 Berlin, Germany.
Anal Biochem. 2016 Jul 1;504:27-9. doi: 10.1016/j.ab.2016.03.019. Epub 2016 Apr 6.
Luciferase-dependent assays, important for biochemical analyses of cytotoxicity and reporter genes, may be perturbed by compounds interfering with the luciferase reaction. We analyzed the impact of different aluminum (Al) species on a luciferase-based assay for determination of cellular adenosine triphosphate. Al(0) nanoparticles (Al(0)-NPs) but not Al2O3-NPs decreased luminescence, correlated to high absorbance of Al(0)-NPs. By contrast, Al ions increased the luminescent signal. Data demonstrate that luciferase-dependent assays can be reciprocally disturbed by Al-NPs or Al ions in a specific manner, depending on the particular Al species. Careful interpretation of data from such experiments is essential in order to obtain conclusive results.
基于荧光素酶的检测对于细胞毒性和报告基因的生化分析很重要，但可能会受到干扰荧光素酶反应的化合物的影响。我们分析了不同铝（Al）物种对基于荧光素酶的细胞三磷酸腺苷测定法的影响。Al(0)纳米颗粒（Al(0)-NPs）而非Al2O3-NPs降低了发光，这与Al(0)-NPs的高吸光度相关。相比之下，Al离子增加了发光信号。数据表明，基于荧光素酶的检测可能会以特定方式被Al-NPs或Al离子反向干扰，具体取决于特定的Al物种。为了获得确凿的结果，对此类实验数据进行仔细解读至关重要。
