锂对卡巴胆碱刺激的大鼠大脑皮层切片中CMP-磷脂酸形成的增强作用及其被肌醇逆转的现象。
Potentiation by lithium of CMP-phosphatidate formation in carbachol-stimulated rat cerebral-cortical slices and its reversal by myo-inositol.
作者信息
Godfrey P P
机构信息
M.R.C. Unit, Radcliffe Infirmary, Oxford, U.K.
出版信息
Biochem J. 1989 Mar 1;258(2):621-4. doi: 10.1042/bj2580621.
Abstract
This paper describes a rapid and simple method for measuring CMP-phosphatidate (CMP-PA; CDP-diacylglycerol), providing a novel assay for inositol phospholipid metabolism. Rat cerebral-cortical slices labelled with [14C]cytidine were incubated with the muscarinic cholinergic agonist carbachol in the presence of various concentrations of LiCl; 10 mM-LiCl greatly enhanced the carbachol-stimulated formation of [14C]CMP-PA over a 60 min incubation period. The potentiation by Li+ was concentration-dependent, with a maximal enhancement at 3 mM and half-maximal enhancement at 0.6 mM-LiCl. The enhancement by Li+ could be reversed by incubation with myo-inositol; a maximal effect was observed with 10 mM-inositol. A similar, though smaller, enhancement of CMP-PA concentrations in the presence of LiCl was observed in slices stimulated with noradrenaline, 5-hydroxytryptamine and K+. The results are discussed in relation to previously observed effects of Li+ on inositol phospholipid metabolism.
摘要
本文描述了一种快速简便的测量CMP-磷脂酸（CMP-PA；CDP-二酰基甘油）的方法，为肌醇磷脂代谢提供了一种新的检测方法。用[14C]胞苷标记的大鼠大脑皮质切片在不同浓度的LiCl存在下与毒蕈碱胆碱能激动剂卡巴胆碱一起孵育；在60分钟的孵育期内，10 mM-LiCl极大地增强了卡巴胆碱刺激的[14C]CMP-PA的形成。Li+的增强作用呈浓度依赖性，在3 mM时最大增强，在0.6 mM-LiCl时半最大增强。Li+的增强作用可通过与肌醇一起孵育而逆转；在10 mM-肌醇时观察到最大效果。在用去甲肾上腺素、5-羟色胺和K+刺激的切片中，在LiCl存在下也观察到了类似但较小的CMP-PA浓度增强。结合先前观察到的Li+对肌醇磷脂代谢的影响对结果进行了讨论。
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本文引用的文献
1
The enzymatic synthesis of inositol phosphatide.肌醇磷脂的酶促合成。
J Biol Chem. 1958 Nov;233(5):1077-83.
2
Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands.锂可增强大脑和唾液腺中激动剂依赖性磷脂酰肌醇反应。
Biochem J. 1982 Sep 15;206(3):587-95. doi: 10.1042/bj2060587.
3
The influence of divalent cations and substrate concentration on the incorporation of myo-inositol into phospholipids of isolated bovine oligodendrocytes.二价阳离子和底物浓度对分离的牛少突胶质细胞中肌醇掺入磷脂的影响。
J Neurochem. 1981 Mar;36(3):868-74. doi: 10.1111/j.1471-4159.1981.tb01674.x.
4
Receptor-mediated metabolism of the phosphoinositides and phosphatidic acid in rat lacrimal acinar cells.大鼠泪腺腺泡细胞中磷酸肌醇和磷脂酸的受体介导代谢
Biochem J. 1984 Feb 15;218(1):187-95. doi: 10.1042/bj2180187.
5
The effects of lithium ion and other agents on the activity of myo-inositol-1-phosphatase from bovine brain.锂离子及其他试剂对牛脑肌醇-1-磷酸酶活性的影响。
J Biol Chem. 1980 Nov 25;255(22):10896-901.
6
Inositol trisphosphate, a novel second messenger in cellular signal transduction.肌醇三磷酸，细胞信号转导中的一种新型第二信使。
Nature. 1984;312(5992):315-21. doi: 10.1038/312315a0.
7
Reduced brain inositol in lithium-treated rats.锂处理大鼠大脑中肌醇减少。
Nat New Biol. 1971 Oct 27;233(43):267-8. doi: 10.1038/newbio233267a0.
8
Lithium-induced reduction in intracellular inositol supply in cholinergically stimulated parotid gland.锂诱导的胆碱能刺激的腮腺细胞内肌醇供应减少。
Biochem J. 1986 Feb 15;234(1):199-204. doi: 10.1042/bj2340199.
9
Lithium inhibits muscarinic-receptor-stimulated inositol tetrakisphosphate accumulation in rat cerebral cortex.锂抑制大鼠大脑皮层中由毒蕈碱受体刺激引起的肌醇四磷酸积累。
Biochem J. 1987 Nov 1;247(3):797-800. doi: 10.1042/bj2470797.
10
Influence of lithium on the positive inotropic effect of phenylephrine and isoprenaline in guinea-pig heart.锂对豚鼠心脏中去氧肾上腺素和异丙肾上腺素正性肌力作用的影响。
Eur J Pharmacol. 1988 May 20;150(1-2):123-9. doi: 10.1016/0014-2999(88)90757-1.
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