Ahlström H, Enblad P, Andersson A, Lörelius L E
Department of Diagnostic Radiology, Uppsala University, Sweden.
Acta Oncol. 1989;28(1):81-6. doi: 10.3109/02841868909111187.
A recently developed experimental model consisting of athymic rats carrying human colonic tumours from the cell line LS 174 T in both hind legs was used. 125I-labelled anti-carcinoembryonic (anti-CEA) monoclonal antibodies were injected either intra-arterially after a bolus injection of mannitol, or intra-peritoneally with or without mannitol. On the fourth day the rats were killed and pieces from the tumours and various organs were measured in a well scintillation counter. Tumour pieces were then submitted to autoradiography and immunohistochemistry for examination of the antibody distribution at the cellular level. In all examined tumours injected with anti-CEA antibodies, most of the antibodies were located in the periphery close to fibrovascular septa. It appears, in addition to the specificity of the antibody for the CEA, that the tumour vascular permeability and anatomy are of utmost importance for tumour targeting in this experimental model with the particular antibody used.
使用了一种最近开发的实验模型，该模型由无胸腺大鼠组成，其两条后腿均携带源自LS 174 T细胞系的人类结肠肿瘤。在静脉注射甘露醇后，将125I标记的抗癌胚抗原（抗CEA）单克隆抗体动脉内注射，或者在有或无甘露醇的情况下腹腔内注射。在第四天处死大鼠，将肿瘤和各种器官的组织块在井型闪烁计数器中进行测量。然后将肿瘤组织块进行放射自显影和免疫组织化学检查，以在细胞水平上检查抗体分布。在所有注射了抗CEA抗体的检查肿瘤中，大多数抗体位于靠近纤维血管间隔的周边。除了抗体对CEA的特异性外，在该实验模型中，使用特定抗体时，肿瘤血管通透性和解剖结构对于肿瘤靶向似乎至关重要。
